Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

Phospho-Lyn (Tyr507) Antibody #2731

Applications Reactivity Sensitivity MW (kDa) Source
W H M (R) Endogenous 53, 56 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-Lyn (Tyr507) Antibody detects endogenous levels of Lyn only when phosphorylated at Tyr507. The antibody may cross-react with phospho-Lck (Tyr505) and phospho-Src (Tyr527) due to high sequence homology.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr507 of human Lyn. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Ramos cells treated with anti-IgM antibody (12 µg/ml for 2 minutes), using Phospho-Lyn (Tyr507) Antibody. The phospho-specificity of the antibody was characterized by treating the membrane without or with calf intestinal alkaline phosphatase (CIP) after western transfer.

Background

Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosine residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family. Autophosphorylation of Tyr396 (equivalent to Tyr416 of Src), located in the catalytic domain, correlates with enzyme activation. Csk-mediated phosphorylation of the carboxy-terminal Tyr507 (equivalent to Tyr527 of Src) inactivates the kinase. Tyrosine phosphorylation and activation of Lyn occurs upon association with cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (2-4). Studies using knockout mice have shown that the net effect of Lyn deficiency is to render B cells hypersensitive to BCR stimulation (5-7), suggesting that the most critical role for Lyn in vivo is in the down-regulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8).

  1. Yamanashi, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 6538-6542.
  2. Yamanashi, Y. et al. (1991) Science 251, 192-194.
  3. Burkhardt, A.L. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 7410-7414.
  4. Ren, C.L. et al. (1994) J. Exp. Med. 179, 673-680.
  5. Wang, J. et al. (1996) J. Exp. Med. 184, 831-838.
  6. Chan, V.W. et al. (1997) Immunity 7, 69-81.
  7. Hibbs, M. L. et al. (1995) Cell 83, 301-311.
  8. Seo, S.J. et al. (2001) J. Immunol. 166, 3710-3723.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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