Cell Signaling Technology

Product Pathways - Development

Numb Antibody #2733

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 72, 74 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Numb Antibody detects endogenous levels of total Numb protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe482 of human Numb. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from A204 and SK-OV-3 cells, using Numb Antibody.

Background

Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling (9).

  1. Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.
  2. Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.
  3. Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.
  4. Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.
  5. Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.
  6. McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203.
  7. Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.
  8. Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.
  9. Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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