Cell Signaling Technology

Product Pathways - DNA Damage

XRCC1 Antibody #2735

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H (Mk) Endogenous 82 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

XRCC1 Antibody detects endogenous levels of total XRCC1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Arg300 of human XRCC1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and K562 cells using XRCC1 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using XRCC1 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).

Background

The X-ray repair cross complementing protein 1 (XRCC1) is a DNA repair protein important in both single strand break repair and base excision repair following damage from ionizing radiation and alkylating agents (1). XRCC1 acts as a scaffold protein to coordinate DNA abasic site repair through interaction with several other repair proteins (2). At least eight XRCC1 protein partners have been identified, including the polynucleotide kinase PNK (3), DNA ligase III (4,5), poly (ADP-ribose) polymerase (6), and PCNA (7). Mutations and polymorphisms in the XRCC1 gene serve as diagnostic markers and are associated with elevated risk of various forms of cancers (8).

  1. Brem, R. and Hall, J. (2005) Nucleic Acids Res. 33, 2512-2520.
  2. Vidal, A.E. et al. (2001) EMBO J. 20, 6530-6539.
  3. Whitehouse, C.J. et al. (2001) Cell 104, 107-117.
  4. Caldecott, K.W. et al. (1994) Mol. Cell. Biol. 14, 68-76.
  5. Nash, R.A. et al. (1997) Biochemistry 36, 5207-5211.
  6. Masson, M. et al. (1998) Mol. Cell. Biol. 18, 3563-3571.
  7. Fan, J. et al. (2004) Nucleic Acids Res. 32, 2193-2201.
  8. Hu, Z. et al. (2005) Cancer Epidemiol. Biomarkers Prev. 14, 1810-1818.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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