Product Pathways - DNA Damage
XRCC1 Antibody #2735
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W IP IF-IC | H | 82 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
XRCC1 Antibody detects endogenous levels of total XRCC1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acids surrounding Arg300 of human XRCC1. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat and K562 cells using XRCC1 Antibody.
IF-IC
Confocal immunofluorescent analysis of HeLa cells using XRCC1 Antibody (green). Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red).
Background
The X-ray repair cross complementing protein 1 (XRCC1) is a DNA repair protein important in both single strand break repair and base excision repair following damage from ionizing radiation and alkylating agents (1). XRCC1 acts as a scaffold protein to coordinate DNA abasic site repair through interaction with several other repair proteins (2). At least eight XRCC1 protein partners have been identified, including the polynucleotide kinase PNK (3), DNA ligase III (4,5), poly (ADP-ribose) polymerase (6), and PCNA (7). Mutations and polymorphisms in the XRCC1 gene serve as diagnostic markers and are associated with elevated risk of various forms of cancers (8).
- Brem, R. and Hall, J. (2005) Nucleic Acids Res. 33, 2512-2520.
- Vidal, A.E. et al. (2001) EMBO J. 20, 6530-6539.
- Whitehouse, C.J. et al. (2001) Cell 104, 107-117.
- Caldecott, K.W. et al. (1994) Mol. Cell. Biol. 14, 68-76.
- Nash, R.A. et al. (1997) Biochemistry 36, 5207-5211.
- Masson, M. et al. (1998) Mol. Cell. Biol. 18, 3563-3571.
- Fan, J. et al. (2004) Nucleic Acids Res. 32, 2193-2201.
- Hu, Z. et al. (2005) Cancer Epidemiol. Biomarkers Prev. 14, 1810-1818.
Application References
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