Product Pathways - DNA Damage
MGMT Antibody #2739
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H Mk | 21 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
MGMT Antibody detects endogenous levels of total MGMT protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acids near the amino terminus of human MGMT. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat and MOLT4 cells using MGMT Antibody.
Background
MGMT (O-6-methylguanine-DNA methyltransferase) is a DNA repair enzyme that participates in a suicide reaction that specifically removes methyl or alkyl groups from the O(6) position of guanine, restoring guanine to its normal form without causing DNA breaks (1). MGMT protects cells from alkylating toxins, and is an important factor in drug resistance to alkylating therapeutic agents (2,3). It is ubiquitously expressed in normal human tissues (4) and is overexpressed in many types of human tumors, but epigenetically silenced in other tumors. MGMT silencing is a marker associated with poor prognosis, but is a good predictive marker for response to alkylating agent chemotherapy (5).
- Pegg, A.E. (2000) Mutat. Res. 462, 83-100.
- Bobola, M.S. et al. (2005) Clin. Cancer Res. 11, 2747-2755.
- Pollack, I.F. et al. (2006) J. Clin. Oncol. 24, 3431-3437.
- Gerson, S.L. et al. (1986) Carcinogenesis 7, 745-749.
- Soejima, H. et al. (2005) Biochem. Cell Biol. 83, 429-437.
Application References
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