Product Pathways - Ca / cAMP / Lipid Signaling
Phospho-MARCKS (Ser152/156) Antibody #2741
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H M R | 80 mouse, rat. 87 human. | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-MARCKS (Ser152/156) Antibody detects endogenous levels of MARCKS only when phosphorylated at serines 152 and 156 .
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH coupled) corresponding to residues surrounding Ser152/156 of rat MARCKS. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 and C6 cells starved for 16 hours and treated with TPA (0.2 µM) for 30 minutes.
Background
Myristoylated alanine-rich protein kinase C substrate (MARCKS) is a major PKC substrate that is distributed in various cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic and mitogenesis (1). PKC phosphorylates Ser152, 156 and 163 of MARCKS (2), which regulates MARCKS's calcium/calmodulin binding activity and filamentous (F)-actin cross-linking activity (3,4). In addition, phosphorylation by PKC results in translocation of MARCKS from membrane to cytoplasm (5).
- Ramsden, J.J. (2000) Int. J. Biochem. Cell Biol. 32, 475-479.
- Heemskerk, F. M. et al. (1993) Biochem. Biophys. Res. Commun. 190, 236-241.
- Graff, J. M. et al. (1989) J. Biol. Chem. 264, 21818-21823.
- Hartwig, J. H. et al. (1992) Nature 356, 618-622.
- Thelen, M. et al. (1991) Nature 351, 320-322.
Application References
- Iwabu, A. et al. (2004) Epidermal growth factor induces fibroblast contractility and motility via a protein kinase C delta-dependent pathway. J. Biol. Chem. 279, 14551-14560. This article references the use of Phospho-MARCKS (Ser152/156) Antibody in the following applications: Western Blotting
- Volmer, R. et al. (2006) PLoS Pathog 2, e19. This article references the use of Phospho-MARCKS (Ser152/156) Antibody in the following applications: Western Blotting
- Park, JA. et al. (2005) Human neutrophil elastase induces hypersecretion of mucin from well-differentiated human bronchial epithelial cells in vitro via a protein kinase C{delta}-mediated mechanism. Am J Pathol. 2005 Sep;167(3):651-61. This article references the use of Phospho-MARCKS (Ser152/156) Antibody in the following applications: Western Blotting
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