Product Pathways - Ca / cAMP / Lipid Signaling
Phospho-MARCKS (Ser152/156) Antibody #2741
PhosphoSitePlus® protein, site, and accession data: MARCKS
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R | Endogenous | 75 mouse, rat. 80 human. | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2741:
- Western Blotting
Specificity / Sensitivity
Phospho-MARCKS (Ser152/156) Antibody detects endogenous levels of MARCKS only when phosphorylated at serines 152 and 156 .
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser152/156 of rat MARCKS. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from NIH/3T3 and C6 cells starved for 16 hours and treated with TPA (0.2 µM) for 30 minutes.
Background
Myristoylated Alanine-Rich C-Kinase Substrate (MARCKS) is a major PKC substrate expressed in many cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic, and mitogenesis (1). PKC phosphorylates Ser159, 163, 167, and 170 of MARCKS in response to growth factors and oxidative stress. Phosphorylation at these sites regulates the calcium/calmodulin binding and filamentous (F)-actin cross-linking activities of MARCKS (2-4). Phosphorylation by PKC also results in translocation of MARCKS from the plasma membrane to the cytoplasm (5).
- Ramsden, J.J. (2000) Int J Biochem Cell Biol 32, 475-9.
- Heemskerk, F.M. et al. (1993) Biochem Biophys Res Commun 190, 236-41.
- Graff, J.M. et al. (1989) J Biol Chem 264, 21818-23.
- Hartwig, J.H. et al. (1992) Nature 356, 618-22.
- Thelen, M. et al. (1991) Nature 351, 320-2.
Application References
- Iwabu, A. et al. (2004) J Biol Chem 279, 14551-60. Applications: Western Blotting
- Volmer, R. et al. (2006) PLoS Pathog 2, e19. Applications: Western Blotting
- Park, J.A. et al. (2005) Am J Pathol 167, 651-61. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
