Product Pathways - Protein Stability
SPINK3 Antibody #2744
PhosphoSitePlus® protein, site, and accession data: Spink3
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | M R | Endogenous | 10 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2744:
- Western Blotting
Specificity / Sensitivity
SPINK3 Antibody detects endogenous levels of total SPINK3 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of the mouse SPINK3 protein. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from rat pancreas and embryonic mouse stages E6.5 and E10.5 using SPINK3 Antibody.
Background
The Kazal type 3 serine peptidase inhibitor (SPINK3) is a serine peptidase inhibitor expressed in the adult pancreas that protects acinar cells from trypsin-induced cell damage (1). Ingestion of milk by newborn SPINK3 knock out mice triggers autophagic cell death in pancreatic acinar cells and the complete disappearance of the pancreas (2). Mutations in the human SPINK3 homolog (known as SPINK1) are associated with chronic pancreatitis, presumably through decreased protection against trypsin-induced autophagic cell death (1,3). SPINK3 may be involved in the embryonic development of several other tissues (4).
- Whitcomb, D.C. et al. (1996) Nat Genet 14, 141-5.
- Ohmuraya, M. et al. (2005) Gastroenterology 129, 696-705.
- Hirota, M. et al. (2006) J Gastroenterol 41, 832-6.
- Wang, J. et al. (2008) Histochem Cell Biol 130, 387-97.
Application References
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Companion Products
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
