Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

FEN-1 Antibody #2746

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 45 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

FEN-1 Antibody detects endogenous levels of total FEN-1 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Lys313 of human FEN-1. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and HeLa cells using FEN-1 Antibody.

Background

Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways (1,2). The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers (3). FEN-1 is also required for non-homologous end joining of double stranded DNA breaks in long patch base excision repair (4,5). The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions (6,7), post-translational modifications (8,9), and subcellular re-localization in response to cell cycle or DNA damage (10).

  1. Shen, B. et al. (2005) Bioessays 27, 717-729.
  2. Liu, Y. et al. (2004) Annu. Rev. Biochem. 73, 589-615.
  3. Sakurai, S. et al. (2005) EMBO J. 24, 683-693.
  4. Wu, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 1303-1308.
  5. Gary, R. et al. (1999) J. Biol. Chem. 274, 4354-4363.
  6. Brosh, R.M. et al. (2001) EMBO J. 20, 5791-5801.
  7. Sharma, S. et al. (2004) J. Biol. Chem. 279, 9847-9856.
  8. Henneke, G. et al. (2003) Oncogene 22, 4301-4313.
  9. Hasan, S. et al. (2001) Mol. Cell 7, 1221-1231.
  10. Qiu, J. et al. (2001) J. Biol. Chem. 276, 4901-4908.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products