Product Pathways - Cell Cycle / Checkpoint
FEN-1 Antibody #2746
| Applications | Reactivity | MW (kDa) | Source |
|---|---|---|---|
| W | H M R Mk | 45 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
FEN-1 Antibody detects endogenous levels of total FEN-1 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acids surrounding Lys313 of human FEN-1. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from Jurkat and HeLa cells using FEN-1 Antibody.
Background
Flap endonuclease-1 (FEN-1) is a structure-specific nuclease with multiple functions in DNA processing pathways (1,2). The replication and DNA repair activities of FEN-1 are critical for genomic stability in the eukaryotic cell. Through interaction with proliferation cell nuclear antigen (PCNA), FEN-1 helps coordinate Okazaki fragment maturation by removing RNA-DNA primers (3). FEN-1 is also required for non-homologous end joining of double stranded DNA breaks in long patch base excision repair (4,5). The multi-functional activities of FEN-1 are regulated by various mechanisms, including protein partner interactions (6,7), post-translational modifications (8,9), and subcellular re-localization in response to cell cycle or DNA damage (10).
- Shen, B. et al. (2005) Bioessays 27, 717-729.
- Liu, Y. et al. (2004) Annu. Rev. Biochem. 73, 589-615.
- Sakurai, S. et al. (2005) EMBO J. 24, 683-693.
- Wu, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 1303-1308.
- Gary, R. et al. (1999) J. Biol. Chem. 274, 4354-4363.
- Brosh, R.M. et al. (2001) EMBO J. 20, 5791-5801.
- Sharma, S. et al. (2004) J. Biol. Chem. 279, 9847-9856.
- Henneke, G. et al. (2003) Oncogene 22, 4301-4313.
- Hasan, S. et al. (2001) Mol. Cell 7, 1221-1231.
- Qiu, J. et al. (2001) J. Biol. Chem. 276, 4901-4908.
Application References
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