Product Pathways - Tyrosine Kinase/ Adaptors
c-Cbl Antibody #2747
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC | H M R Mk (B) | Endogenous | 120 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
This antibody detects endogenous levels of total c-Cbl protein. The antibody may also cross-react with Cbl-b protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of human c-Cbl. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of lysates from MCF-7, Raji, THP-1, RAW, COS and H-4-II-E cells, using c-CBL antibody.
IF-IC
Immunofluorescent staining of paraformaldehyde-fixed RAW cells, using c-CBL antibody.
Background
The c-Cbl proto-oncogene is a ubiquitously expressed cytoplasmic adaptor protein that is especially predominant in hematopoietic cells (1,2). c-Cbl is rapidly tyrosine-phosphorylated in response to stimulation of a variety of cell-surface receptors and becomes associated with a number of intracellular signaling molecules such as protein tyrosine kinases, phosphatidylinositol-3 kinase, Crk and 14-3-3 proteins (3,4). c-Cbl possesses a highly conserved amino-terminal phosphotyrosine binding domain (TKB) and a C3HC4 RING finger motif. The TKB recognizes phosphorylated tyrosines on activated receptor tyrosine kinases (RTKs) as well as other nonreceptor tyrosine kinases. Its RING finger domain recruits ubiquitin-conjugating enzymes. These two domains are primarily responsible for c-Cbl's ubiquitin ligase activity and downregulation of RTKs (3). In human cancer tissues, c-Cbl is frequently tyrosine-phosphorylated in a tumor-specific manner (5). Phosphorylation of Tyr731 of c-Cbl provides a docking site for downstream signaling components such as p85 and Fyn (6).
- Blake, T.J. et al. (1991) Oncogene 6, 653-657.
- Thien, C.B. and Langdon, W.Y. (1998) Immunol. Cell Biol. 76, 473-482.
- Christine, B.F. et al. (2001) Nat. Rev. Mol. Cell Biol. 2, 294-307.
- Feshchenko, E.A. et al. (1998) J. Biol. Chem. 273, 8323-8331.
- Kamei, T. et al. (2000) Int. J. Oncol. 17, 335-339.
- Hunter, C. et al. (1999) J. Biol. Chem. 274, 2097-2106.
Application References
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Companion Products
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- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
