Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

Sox2 Antibody #2748

Applications Reactivity MW (kDa) Source
W IP H M 35 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Sox2 Antibody detects endogenous levels of total Sox2 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acids near the carboxy terminus of human Sox2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NCCIT and NTERA2 cells using Sox2 Antibody.

Background

Embryonic stem cells are derived from the inner cell mass of the blastocyst, and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of genes that is crucial for the maintenance of pluripotency (1). Sox2, Oct-4 and Nanog co-operate in this network (1-3), and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (6,7).

  1. Nichols, J. et al. (1998) Cell 95, 379-391.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17, 126-140.
  3. Rodda, D.J. et al. (2005) J. Biol. Chem. 280, 24731-24737.
  4. Matin, M.M. et al. (2004) Stem Cells 22, 659-668.
  5. Niwa, H. et al. (2000) Nat. Genet. 24, 372-376.
  6. Boyer, L.A. et al. (2005) Cell 122, 947-956.
  7. Loh, Y.H. et al. (2006) Nat. Genet. 38, 431-440.

Application References

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