Cell Signaling Technology

Product Pathways - Wnt / Hedgehog / Notch

Oct-4 Antibody #2750

Applications Reactivity MW (kDa) Source
W IHC-P IF-IC F H 45 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Oct-4 Antibody detects endogenous levels of total Oct-4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to amino acids near the carboxy terminus of human Oct-4. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of NCCIT and NTERA2 cell extracts using Oct-4 Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human seminoma (left) or normal testis (right), showing nuclear localization using Oct-4 Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of untreated NCCIT cells using Oct-4 Antibody (blue) compared to a nonspecific negative control antibody (red).


IF-IC

IF-IC

Confocal immunofluorescent analysis of NCCIT cells using Oct-4 Antibody (green) and β-Tubulin (9F3) Rabbit mAb (Alexa Fluor® 555 Conjugate) #2116 (red).

Background

Oct-4 (POU5F1) is a transcription factor expressed in undifferentiated embryonic stem cells and embryonic germ cells (1). A network of key factors that includes Oct-4, Nanog, and Sox2 is necessary for the maintenance of pluripotent potential. Downregulation of Oct-4 has been shown to trigger cell differentiation (2,3). Oct-4 is a useful germ cell tumor marker (4).

  1. Looijenga, L.H. et al. (2003) Cancer Res. 63, 2244-2250.
  2. Pesce, M. and Scholer, H.R. (2001) Stem Cells 19, 271-278.
  3. Pan, G. and Thomson, J.A. (2007) Cell Res. 17, 42-49.
  4. Cheng, L. et al. (2007) J. Pathol. 211, 1-9.

Application References

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