Cell Signaling Technology

Product Pathways - Tyrosine Kinase / Adaptors

Fgr Antibody #2755

Applications Reactivity Sensitivity MW (kDa) Source
W IP F H Endogenous 56 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Fgr Antibody detects endogenous levels of total Fgr proteins. This antibody does not cross-react with other proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues close to the amino terminus of human Fgr. Antibodies are purified by protein A and peptide affinity chromatography.  

Western Blotting

Western Blotting

Western blot analysis of Raji cell lysate, using Fgr Antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Raji cells, using Fgr Antibody (blue) compared to a nonspecific negative control antibody (red).

Background

Fgr is a member of the Src tyrosine kinase family. It has a membrane-associated amino-terminal domain that is highly divergent from other family members, internal conserved SH2 and SH3 domains and a highly conserved carboxy-terminal tyrosine kinase catalytic domain (1,2). Tyrosine 412 is located in the activation loop, and phosphorylation of this residue is critical for the activation of Fgr tyrosine kinase activity. c-Fgr is predominantly expressed in cells of hematopoietic origin including differentiated myeloid cells, NK and B cells (3,4). Fgr plays an important role in the signaling cascade from membrane receptors lacking intrinsic tyrosine kinase activity such as Bcr, FcR, and the integrin family of receptors (5). It was demonstrated that Fgr functions as a selective inhibitor of beta2 integrin-mediated signaling and Syk kinase function in monocytes (5).

  1. Willman, C.L. et al. (1991) Blood 77, 726-734.
  2. Patel, M. et al. (1991) Pathobiology 59, 289-292.
  3. Wechsler, R.J. and Monroe, J.G. (1995) J. Immunol. 154, 3234-3244.
  4. Notario, V. et al. (1989) J. Cell Biol. 109, 3129-3136.
  5. Vines, C.M. et al. (2001) Immunity 15, 507-519.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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