Cell Signaling Technology

Product Pathways - Protein Stability

CUL3 Antibody #2759

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Mk Endogenous 82, 84 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

CUL3 Antibody detects endogenous levels of total Cullin-3.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu750 of human Cullin-3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lysates, using CUL3 Antibody.

Background

CUL3 (Cullin-3) is a member of the cullin-based ubiquitin ligase family. By interacting with Hrt1 and BTB domain containing proteins, the complex functions as a CUL3-based E3 ligase to bring specific substrates to ubiquitinylation and degradation (1). The CUL3 complex has been shown to target many substrates involved in cell cycle progression (2), transcription (3), development and differentiation (4,5).

  1. Pintard, L. et al. (2004) EMBO J. 23, 1681-1687.
  2. Singer, J.D. et al. (1999) Genes Dev. 13, 2375-2387.
  3. Furukawa, M. and Xiong, Y. (2005) Mol Cell Biol 25, 162-171.
  4. Zhu, S. et al. (2005) J. Neurosci. 25, 4189-4197.
  5. Ou, C.Y. et al. (2002) Genes Dev. 16, 2403-2414.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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