Product Pathways - Glucose Metabolism
Glucagon Antibody #2760
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| IHC-P IHC-F IF-F | H M R | Endogenous | 4 | Rabbit |
Applications Key:
IHC-P=Immunohistochemistry (Paraffin)
IHC-F=Immunohistochemistry (Frozen)
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Glucagon Antibody detects endogenous levels of total glucagon protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence of human glucagon. Antibodies are purified by protein A and peptide affinity chromatography.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human pancreas, showing staining of α cells, using Glucagon Antibody.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded mouse pancreas, showing staining of α cells, using Glucagon Antibody.
IHC-F (frozen)
Immunohistochemical analysis of frozen mouse pancreas using Glucagon Antibody.
IF-F
Confocal immunofluorescent analysis of normal mouse pancreas using Glucagon Antibody (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Glucose homeostasis is regulated by a variety of hormones including glucagon. Glucagon is synthesized as the precursor molecule proglucagon and is proteolytically processed to yield the mature peptide in α cells of the pancreatic islets. Glucagon causes the release of glucose from glycogen and stimulates gluconeogenesis in the liver (1,2).
- Hers, H.G. and Hue, L. (1983) Annu. Rev. Biochem. 52, 617-653.
- Granner, D. and Pilkis, S. (1990) J. Biol. Chem. 265, 10173-10176.
Application References
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Companion Products
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
