Cell Signaling Technology

Product Pathways - Development

Numb (C44B4) Rabbit mAb #2761

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IF-IC F H Endogenous 72, 74 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Numb (C44B4) Rabbit mAb detects endogenous levels of Numb protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Phe482 of human Numb protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa and SK-OV-3 cells using Numb (C44B4) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of A-204 cells using Numb (C44B4) Rabbit mAb (blue) compared to a nonspecific negative control antibody (red).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using Numb (C44B4) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


Background

Numb contains an amino-terminal phosphotyrosine-binding (PTB) domain and carboxy-terminal endocytic binding motifs for α-adaptin and EH (Eps15 homology) domain-containing proteins, indicating a role in endocytosis (1,2). There are four mammalian Numb splicing isoforms that are differentially expressed and may have distinct functions (3-5). Numb acts as a negative regulator of Notch signaling by promoting ubiquitination and degradation of Notch (6). The protein is asymmetrically segregated into one daughter cell during cell division, producing two daughter cells with different responses to Notch signaling and different cell fates (7,8). The localization of Numb can also be regulated by G-protein coupled receptor (GPCR) and PKC signaling (9).

  1. Berdnik, D. et al. (2002) Dev. Cell 3, 221-231.
  2. Santolini, E. et al. (2000) J. Cell Biol. 151, 1345-1352.
  3. Dho, S.E. et al. (1999) J. Biol. Chem. 274, 33097-33104.
  4. Verdi, J.M. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 10472-10476.
  5. Toriya, M. et al. (2006) Dev. Neurosci. 28, 142-155.
  6. McGill, M.A. and McGlade, C.J. (2003) J. Biol. Chem. 278, 23196-23203.
  7. Verdi, J.M. et al. (1996) Curr. Biol. 6, 1134-1145.
  8. Reugels, A.M. et al. (2006) Dev. Dyn. 235, 934-948.
  9. Dho, S.E. et al. (2006) Mol. Biol. Cell 17, 4142-4155.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products