Cell Signaling Technology

Product Pathways - Apoptosis

Bcl-xL Antibody #2762

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 30 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Bcl-xL Antibody detects endogenous levels of total Bcl-xL protein. The antibody does not cross-react with other Bcl-2 family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat (human), NIH/3T3 (mouse) or C6 (rat) cells, untreated or etoposide-treated (25 µM, Jurkat) or staurosporine-treated (1 µM, NIH/3T3 and C6), using Bcl-xL Antibody.

IP

IP

Immunoprecipitation of Bcl-xL from Jurkat cell extracts, using Bcl-xL Antibody. Lane 1 is the lysate control; lane 2 is antibody alone and lane 3 is antibody plus lysate.

Background

Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5).

  1. Adams, J.M. and Cory, S. (1998) Science 281, 1322-1326.
  2. Minn, A.J. et al. (1999) EMBO. J. 18, 632-643.
  3. Vander Heiden, M.G. et al. (2001) J. Biol. Chem. 276, 19414-19419.
  4. Fan, M. et al. (2000) J. Biol. Chem. 275, 29980-29985.
  5. Poruchynsky, M.S. et al. (1998) Cancer Res. 58, 3331-3338.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products


For Research Use Only. Not For Use In Diagnostic Procedures.

Products