Cell Signaling Technology

Product Pathways - Development

MESDC2 (C22F5) Rabbit mAb #2763

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H M R Mk Endogenous 25, 26 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MESDC2 (C22F5) Rabbit mAb detects endogenous levels of total MESDC2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant human MESDC2 protein.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell lines using MESDC2 (C22F5) Rabbit mAb.

Background

Mesoderm development candidate genes 1 (MESDC1) and 2 (MESDC2) were identified from the mesoderm development (MESD) deletion interval located on mouse chromosome 7 (1). MESD acts as a chaperone for low-density lipoprotein receptor (LDLR) proteins in the ER, and particularly associates with Wnt signaling pathway coreceptors LRP5 and LPR6 (2). MESD is required for proper LRP5/6 folding and maturation to the cell surface, which plays a major role in Wnt signaling (3-5). The interaction between MESD and LRP5 is disrupted by a G171V mutation in LRP5 that is found in individuals with a familial phenotype characterized by high bone mass (6).

  1. Wines, M.E. et al. (2001) Genomics 72, 88-98.
  2. Hsieh, J.C. et al. (2003) Cell 112, 355-67.
  3. Herz, J. and Marschang, P. (2003) Cell 112, 289-92.
  4. Li, Y. et al. (2006) FEBS Lett 580, 5423-8.
  5. Li, Y. et al. (2005) J Cell Sci 118, 5305-14.
  6. Zhang, Y. et al. (2004) Mol Cell Biol 24, 4677-84.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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