Product Pathways - Translational Control
PKR (N216) Antibody #2766
PhosphoSitePlus® protein, site, and accession data: PKR
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H | Endogenous | 74 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2766:
- Western Blotting
Specificity / Sensitivity
PKR (N216) Antibody detects endogenous levels of total PKR protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human PKR. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell lines, using PKR (N216) Antibody.
Background
Protein kinase R (PKR) is transcriptionally induced by interferon and activated by double-stranded RNA (dsRNA). PKR inhibits translation initiation through phosphorylation of the alpha subunit of the initiation factor eIF2 (eIF2alpha) and also controls the activation of several transcription factors such as NF-kappaB, p53 and the Stats. In addition, PKR mediates apoptosis induced by many different stimuli, such as LPS, TNF-alpha, viral infection and serum starvation (1,2). Activation of PKR by dsRNA results in PKR dimerization and autophosphorylation at threonine 446 and threonine 451 in the activation loop. Substitution of threonine 451 to alanine completely inactivates PKR, while a mutant with a threonine 446 to alanine substitution was partially active (3). Recently, activation of PKR has been implicated in the pathologies of neurodegenerative diseases, including Alzheimer disease (4,5).
- Williams, B.R. (1999) Oncogene 18, 6112-6120.
- Gil, J. and Esteban, M. (2000) Apoptosis 5, 107-114.
- Romano, P. R. et al. (1998) Mol. Cell. Biol. 18, 2282-2297.
- Peel, A.L. and Bredesen, D.E. (2003) Neurobiol. Dis. 14, 52-62.
- Peel, A.L. (2004) J. Neuropath. Exp. Neurol. 63, 97-105.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
