Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

Bcl-xL (54H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) #2767

Applications Reactivity Sensitivity Isotype
F H M R Endogenous Rabbit IgG

Applications Key:  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Bcl-xL (54H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total Bcl-xL protein. The antibody does not cross-react with other Bcl-2 family members.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of SEM (blue) and K-562 (green) cells using Bcl-xL (54H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) compared to nonspecific Rabbit IgG Isotype Control (Alexa Fluor® 488 Conjugate) #4340 (red).

Description

This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated antibody #2764 reacts with human, mouse, rat and monkey Bcl-xL protein. CST expects that Bcl-xL (54H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize Bcl-xL in these species.

Background

Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5).

  1. Adams, J.M. and Cory, S. (1998) Science 281, 1322-1326.
  2. Minn, A.J. et al. (1999) EMBO. J. 18, 632-643.
  3. Vander Heiden, M.G. et al. (2001) J. Biol. Chem. 276, 19414-19419.
  4. Fan, M. et al. (2000) J. Biol. Chem. 275, 29980-29985.
  5. Poruchynsky, M.S. et al. (1998) Cancer Res. 58, 3331-3338.

Application References

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Companion Products

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.Alexa Fluor® is a registered trademark of Molecular Probes, Inc.

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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