Product Pathways - Apoptosis
Bcl-xL (54H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) #2767
|2767S||100 µl (50 tests)||---||In Stock||---|
|2767||carrier free and custom formulation / quantity||email request|
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|F||1:50||Human, Mouse, Rat, Monkey||Endogenous||Rabbit IgG|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: F=Flow Cytometry
Specificity / Sensitivity
Bcl-xL (54H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total Bcl-xL protein. The antibody does not cross-react with other Bcl-2 family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-6.
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometric analysis of human cells. The unconjugated antibody #2764 reacts with human, mouse, rat and monkey Bcl-xL protein. CST expects that Bcl-xL (54H6) Rabbit mAb (Alexa Fluor® 488 Conjugate) will also recognize Bcl-xL in these species.
Bcl-xL prevents apoptosis through two different mechanisms: heterodimerization with an apoptotic protein inhibits its apoptotic effect (1,2) and formation of mitochondrial outer membrane pores help maintain a normal membrane state under stressful conditions (3). Bcl-xL is phosphorylated by JNK following treatment with microtubule-damaging agents such as paclitaxel, vinblastine and nocodazole (4,5).
- Adams, J.M. and Cory, S. (1998) Science 281, 1322-1326.
- Minn, A.J. et al. (1999) EMBO. J. 18, 632-643.
- Vander Heiden, M.G. et al. (2001) J. Biol. Chem. 276, 19414-19419.
- Fan, M. et al. (2000) J. Biol. Chem. 275, 29980-29985.
- Poruchynsky, M.S. et al. (1998) Cancer Res. 58, 3331-3338.
- Mirlashari, M.R. et al. (2012) Leuk Res 36, 499-508.
- Monticelli, L.A. et al. (2009) Proc Natl Acad Sci U S A 106, 19461-6. Applications: Flow Cytometry.
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For Research Use Only. Not For Use In Diagnostic Procedures.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patents No. 5,675,063 and 7,429,487) from Epitomics, Inc.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc., for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.