Cell Signaling Technology

Product Pathways - Apoptosis / Autophagy

Bax Antibody #2772

Applications Reactivity MW (kDa) Source
W IP H M R Mk 20 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Bax Antibody detects endogenous levels of total Bax protein. The antibody does not cross-react with other Bcl-2 family members.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the amino-terminal residues of human Bax. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from 293 (human), COS (monkey), L929 (mouse) and PC12 (rat) cells, using Bax Antibody.

IP

IP

Immunoprecipitation of Bax from Jurkat cell extract, using Bax Antibody. Lane 1 is the lysate control; lane 2 is antibody alone; lane 3 is antibody plus lysate.

Background

Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (3,4).

  1. Wei, M.C. et al. (2001) Science 292, 727-730.
  2. Jurgensmeier, J.M. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 4997-5002.
  3. Narita, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 14681-14686.
  4. Marzo, I. et al. (1998) Science 281, 2027-2031.

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