Product Pathways - Apoptosis
Bax Antibody #2772
|2772S||100 µl (10 western blots)||---||In Stock||---|
|2772||carrier free and custom formulation / quantity||email request|
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|W||1:1000||Human, Mouse, Rat, Monkey||Endogenous||20||Rabbit|
Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation
Specificity / Sensitivity
Bax Antibody detects endogenous levels of total Bax protein. The antibody does not cross-react with other Bcl-2 family members.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human Bax. Antibodies are purified by protein A and peptide affinity chromatography.
Western blot analysis of extracts from 293 (human), COS (monkey), L929 (mouse) and PC12 (rat) cells, using Bax Antibody.
Western blot analysis of extracts from HeLa cells, transfected with 100 nM SignalSilence® Control siRNA (Fluorescein Conjugate) #6201 (-), SignalSilence® Bax siRNA I (+) or SignalSilence® Bax siRNA II (+), using Bax Antibody and α-Tubulin (11H10) Rabbit mAb #2125. The Bax Antibody confirms silencing of Bax expression and α-Tubulin (11H10) Rabbit mAb is used to control for loading and specificity of Bax siRNA.
Immunoprecipitation of Bax from Jurkat cell extract, using Bax Antibody. Lane 1 is the lysate control; lane 2 is antibody alone; lane 3 is antibody plus lysate.
Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (3,4).
- Xie, S. et al. (2001) J. Biol. Chem. 276 (39), 36194-36199. Applications: Western Blotting.
- Yusta, B. et al. (2002) J Biol Chem 277, 24896-906. Applications: Western Blotting.
- Leu, J. I. et al. (2004) Nat. Cell. Biol. 6, 443-450. Applications: Western Blotting, IP.
- Cheong, J. W. et al. (2003) Clin. Cancer Res. 9, 5018-5027. Applications: Western Blotting.
- Rusinol, A. E. et al. (2004) J. Biol. Chem. 279, 1392-1399. Applications: Western Blotting.
- Widenmaier, S.B. et al. (2009) J Biol Chem 284, 30372-82. Applications: Western Blotting.
- Ikner, A. and Ashkenazi, A. (2011) J Biol Chem 286, 21546-54. Applications: Western Blotting.
- Pritchard, J.R. et al. (2011) Cancer Res 71, 5850-8. Applications: Western Blotting.
- Lu, W.J. et al. (2011) Cell Death Differ 18, 1046-56. Applications: Western Blotting.
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For Research Use Only. Not For Use In Diagnostic Procedures.
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