Product Pathways - Apoptosis

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Bax Antibody #2772

Applications	Reactivity	Sensitivity	MW (kDa)	Source
W IP	H M R Mk	Endogenous	20	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

2772:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Bax Antibody detects endogenous levels of total Bax protein. The antibody does not cross-react with other Bcl-2 family members.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal residues of human Bax. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (3,4).

1. Wei, M.C. et al. (2001) Science 292, 727-730.
2. Jurgensmeier, J.M. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 4997-5002.
3. Narita, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 14681-14686.
4. Marzo, I. et al. (1998) Science 281, 2027-2031.

Application References

• Xie, S. et al. (2001) Reactive oxygen species-induced phosphorylation of p53 on serine 20 is mediated in part by Polo-like kinase-3. J. Biol. Chem. 276 (39), 36194-36199. Applications: Western Blotting
• Yusta, B. et al. (2002) J Biol Chem 277, 24896-906. Applications: Western Blotting
• Leu, J. I. et al. (2004) Mitochondrial p53 activates Bak and causes disruption of a Bak-Mcl1 complex. Nat. Cell. Biol. 6, 443-450. Applications: IP Western Blotting
• Cheong, J. W. et al. (2003) Induction of apoptosis by apicidin, a histone deacetylase inhibitor, via the activation of mitochondria-dependent caspase cascades in human Bcr-Abl-positive leukemia cells. Clin. Cancer Res. 9, 5018-5027. Applications: Western Blotting
• Rusinol, A. E. et al. (2004) AKT/protein kinase B regulation of BCL family members during oxysterol-induced apoptosis. J. Biol. Chem. 279, 1392-1399. Applications: Western Blotting
• Widenmaier, S.B. et al. (2009) J Biol Chem 284, 30372-82. Applications: Western Blotting
• Ikner, A. and Ashkenazi, A. (2011) J Biol Chem 286, 21546-54. Applications: Western Blotting
• Pritchard, J.R. et al. (2011) Cancer Res 71, 5850-8. Applications: Western Blotting
• Lu, W.J. et al. (2011) Cell Death Differ 18, 1046-56. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 6321 SignalSilence^® Bax siRNA I
• 2762 Bcl-xL Antibody
• 4592 Bik Antibody
• 9292 Bad Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

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For Research Use Only. Not For Use In Diagnostic Procedures.