Product Pathways - Apoptosis / Autophagy

Bax Antibody #2772

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• application references
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Applications	Reactivity	MW (kDa)	Source
W IP	H M R Mk	20	Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Bax Antibody detects endogenous levels of total Bax protein. The antibody does not cross-react with other Bcl-2 family members.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the amino-terminal residues of human Bax. Antibodies are purified by protein A and peptide affinity chromatography.

Background

Bax is a key component for cellular induced apoptosis through mitochondrial stress (1). Upon apoptotic stimulation, Bax forms oligomers and translocates from the cytosol to the mitochondrial membrane (2). Through interactions with pore proteins on the mitochondrial membrane, Bax increases the membrane's permeability, which leads to the release of cytochrome c from mitochondria, activation of caspase-9 and initiation of the caspase activation pathway for apoptosis (3,4).

1. Wei, M.C. et al. (2001) Science 292, 727-730.
2. Jurgensmeier, J.M. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 4997-5002.
3. Narita, M. et al. (1998) Proc. Natl. Acad. Sci. USA 95, 14681-14686.
4. Marzo, I. et al. (1998) Science 281, 2027-2031.

Application References

• Xie, S. et al. (2001) Reactive oxygen species-induced phosphorylation of p53 on serine 20 is mediated in part by Polo-like kinase-3. J. Biol. Chem. 276 (39), 36194-36199. This article references the use of Bax Antibody in the following applications: Western Blotting
• Yusta, B. et al. (2002) Glucagon-like peptide-2 receptor activation engages Bad and glycogen synthase kinase-3 in a protein kinase A-dependent manner and prevents apoptosis following inhibition of phosphatidylinositol 3-kinase. J. Biol. Chem. 277 (28), 24896-24906. This article references the use of Bax Antibody in the following applications: Western Blotting
• Leu, J. I. et al. (2004) Mitochondrial p53 activates Bak and causes disruption of a Bak-Mcl1 complex. Nat. Cell. Biol. 6, 443-450. This article references the use of Bax Antibody in the following applications: IP Western Blotting
• Cheong, J. W. et al. (2003) Induction of apoptosis by apicidin, a histone deacetylase inhibitor, via the activation of mitochondria-dependent caspase cascades in human Bcr-Abl-positive leukemia cells. Clin. Cancer Res. 9, 5018-5027. This article references the use of Bax Antibody in the following applications: Western Blotting
• Rusinol, A. E. et al. (2004) AKT/protein kinase B regulation of BCL family members during oxysterol-induced apoptosis. J. Biol. Chem. 279, 1392-1399. This article references the use of Bax Antibody in the following applications: Western Blotting

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