Product Pathways - Apoptosis / Autophagy
Phospho-PEA-15 (Ser104) Antibody #2776
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H R (M) | Endogenous | 15 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-PEA-15 (Ser104) Antibody detects endogenous levels of PEA-15 only when phosphorylated at serine 104.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser104 of human PEA-15. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from serum starved human A172 and rat C6 cells, untreated or treated with 200 nM TPA, using Phospho-PEA-15 (Ser104) Antibody (upper) or PEA-15 Antibody (lower).
Background
PEA-15 is a 15 kDa phosphoprotein expressed abundantly in astrocytes and fibroblasts as well as tissues including lung and eye (1). The protein has been shown to coordinate cell growth, death and glucose ultilization (2-4). The amino-terminal DED domain of PEA-15 mediates its binding to FADD or Erk and futher regulates the Erk and apoptosis pathways. PEA-15 can be phosphorylated at two serine residues, Ser104 and Ser116, located within the carboxy terminus. Phosphorylation at these sites regulates binding to Erk and FADD (2,3).
- Danziger, N. et al. (1995) J. Neurochem. 64, 1016-1025.
- Krueger, J. et al. (2005) Mol. Biol. Cell 16, 3552-3561.
- Renganathan, H. et al. (2005) Biochem. J. 390, 729-735.
- Condorelli, G. et al. (1998) EMBO J. 17, 3858-3866.
Application References
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Companion Products
- 2780 PEA-15 Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
