Cell Signaling Technology

Product Pathways - Apoptosis

PEA-15 Antibody #2780

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 15 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

PEA-15 Antibody detects endogenous levels of total PEA-15 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding residue surrounding Leu60 of human PEA-15. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A172, Neuro2A, C6 and COS cells, using PEA-15 Antibody.

Background

PEA-15 is a 15 kDa phosphoprotein expressed abundantly in astrocytes and fibroblasts as well as in tissues, including the lung and eye (1). The protein has been shown to coordinate cell growth, death, and glucose ultilization (2-4). The amino-terminal DED domain of PEA-15 mediates its binding to FADD or Erk and futher regulates the Erk and apoptosis signaling pathways. PEA-15 can be phosphorylated at two serine residues, Ser104 and Ser116, located within the carboxy terminus. Phosphorylation at these sites regulates binding to Erk and FADD (2,3).

  1. Danziger, N. et al. (1995) J. Neurochem. 64, 1016-1025.
  2. Krueger, J. et al. (2005) Mol. Biol. Cell 16, 3552-3561.
  3. Renganathan, H. et al. (2005) Biochem. J. 390, 729-735.
  4. Condorelli, G. et al. (1998) EMBO J. 17, 3858-3866.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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