Cell Signaling Technology

Product Pathways - Apoptosis

Phospho-FADD (Ser194) Antibody (Human Specific) #2781

Applications Reactivity Sensitivity MW (kDa) Source
W H Endogenous 28 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Phospho-FADD (Ser194) Antibody (Human Specific) detects endogenous levels of human FADD protein only when phosphorylated at serine 194.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser194 of human FADD. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from Jurkat and HeLa cells, hydroxyurea-treated (4 mM, 20 hours) or nocodazole-treated (1 µg/ml, 20 hours), using Phospho-FADD Ser194 Antibody (Human Specific) (upper) or control FADD Antibody (Human Specific) #2782 (lower).

Background

Fas-associated death domain (FADD or Mort 1) functions as an important adaptor in coupling death signaling from membrane receptors, such as the Fas ligand and TNF family (DR3, DR4 and DR5), to caspase-8 (1,2). FADD has a carboxy-terminal death domain, which interacts with the cytoplasmic tail of the membrane receptor, and an amino-terminal death effector domain, which interacts with caspase-8. Clustering of the receptors upon stimulation brings about FADD and caspase-8 oligomerization, activating the caspase signaling pathway. Human FADD is phosphorylated mainly at Ser194, while mouse FADD is phosphorylated at Ser191. In both cases, the phosphorylation is cell cycle-dependent (3) and may be related to its regulatory role in embryonic development and cell cycle progression (4,5).

  1. Ashkenazi, A. and Dixit, V.M. (1998) Science 281, 1305-1308.
  2. Kuang, A. A. et al. (2000) J. Biol. Chem. 275, 25065-25068.
  3. Scaffidi, C. et al. (2000) J. Immunol. 164, 1236-1242.
  4. Newton, K. et al. (2000) EMBO J. 19, 931-941.
  5. Zhang, J. et al. (2001) J. Biol. Chem. 276, 29815-29818.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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