Product Pathways - Neuroscience
Phospho-Tyrosine Hydroxylase (Ser40) Antibody #2791
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP IF-IC E-P | H R (H) (M) | Endogenous | 55 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IF-IC=Immunofluorescence (Immunocytochemistry)
E-P=ELISA (Peptide)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-Tyrosine Hydroxylase (Ser40) Antibody detects endogenous levels of tyrosine hydroxylase only when phosphorylated at serine 40.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) derived from the sequence surrounding Ser40 of human tyrosine hydroxylase. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from PC12 cells, untreated or forskolin-treated (30 µM), using Phospho-Tyrosine Hydroxylase (Ser40) Antibody (upper) or Tyrosine Hydroxylase Antibody #2792 (lower).
IF-IC
Confocal immunofluorescent images of PC-12 cells, untreated (A,C) and calf intestinal phosphatase treated (B), labeled with Phospho-Tyrosine Hydroxylase (Ser40) Antibody #2791 (green, top) and Tyrosine Hydroxylase Antibody #2792 (green, bottom) compared to an isotype control (D). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).
Background
Tyrosine hydroxylase catalyzes the rate-limiting step in the synthesis of dopamine and other catecholamines. The enzyme's activity is regulated at the levels of transcription, translation and posttranslational modification (1,2). Tyrosine hydroxylase exists in several different isoforms, ranging from 50 kDa to about 62 kDa in humans. The amino-terminal regulatory domain contains three phospho-serine sites: Ser9, Ser31 and Ser40 (4,5). Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of the enzyme (4-6).
- Kumer, S.C. and Vrana, K.E. (1996) J. Neurochem. 67, 443-462.
- Bodeau-Pean, S. et al. (1999) J. Biol. Chem. 274, 3469-3475.
- Alterio, J. et al. (1998) J. Biol. Chem. 273, 10196-10201.
- Lew, J. Y. et al. (1999) Mol. Pharmacol. 55, 202-209.
- Vie, A. et al. (1999) J. Biol. Chem. 274, 16788-16795.
- Lindgren, N. et al. (2000) J. Neurochem. 74, 2470-2477.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2792 Tyrosine Hydroxylase Antibody
- 1132 Tyrosine Hydroxylase (Ser40) Biotinylated Peptide
- 7660 HTScan® MAPKAPK-5 Kinase Assay Kit
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
