Cell Signaling Technology

Product Pathways - Neuroscience

Phospho-Tyrosine Hydroxylase (Ser40) Antibody #2791

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC E-P H R (H) (M) Endogenous 55 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  E-P=ELISA (Peptide)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-Tyrosine Hydroxylase (Ser40) Antibody detects endogenous levels of tyrosine hydroxylase only when phosphorylated at serine 40.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) derived from the sequence surrounding Ser40 of human tyrosine hydroxylase. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from PC12 cells, untreated or forskolin-treated (30 µM), using Phospho-Tyrosine Hydroxylase (Ser40) Antibody (upper) or Tyrosine Hydroxylase Antibody #2792 (lower).

IF-IC

IF-IC

Confocal immunofluorescent images of PC-12 cells, untreated (A,C) and calf intestinal phosphatase treated (B), labeled with Phospho-Tyrosine Hydroxylase (Ser40) Antibody #2791 (green, top) and Tyrosine Hydroxylase Antibody #2792 (green, bottom) compared to an isotype control (D). Blue pseudocolor = DRAQ5® (fluorescent DNA dye).

Background

Tyrosine hydroxylase catalyzes the rate-limiting step in the synthesis of dopamine and other catecholamines. The enzyme's activity is regulated at the levels of transcription, translation and posttranslational modification (1,2). Tyrosine hydroxylase exists in several different isoforms, ranging from 50 kDa to about 62 kDa in humans. The amino-terminal regulatory domain contains three phospho-serine sites: Ser9, Ser31 and Ser40 (4,5). Phosphorylation at Ser40 by PKA positively regulates the catalytic activity of the enzyme (4-6).

  1. Kumer, S.C. and Vrana, K.E. (1996) J. Neurochem. 67, 443-462.
  2. Bodeau-Pean, S. et al. (1999) J. Biol. Chem. 274, 3469-3475.
  3. Alterio, J. et al. (1998) J. Biol. Chem. 273, 10196-10201.
  4. Lew, J. Y. et al. (1999) Mol. Pharmacol. 55, 202-209.
  5. Vie, A. et al. (1999) J. Biol. Chem. 274, 16788-16795.
  6. Lindgren, N. et al. (2000) J. Neurochem. 74, 2470-2477.

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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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