Product Pathways - Metabolism
SCD1 (C12H5) Rabbit mAb #2794
|2794S||100 µl (10 western blots)||---||In Stock||---|
|2794||carrier free and custom formulation / quantity||email request|
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Species cross-reactivity is determined by western blot.
Applications Key: W=Western Blotting, IP=Immunoprecipitation, IHC-P=Immunohistochemistry (Paraffin), IF-IC=Immunofluorescence (Immunocytochemistry)
Specificity / Sensitivity
SCD1 (C12H5) Rabbit mAb detects endogenous levels of total SCD1 protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues surrounding Leu35 of mouse SCD1.
Immunohistochemical analysis of paraffin-embedded 3T3-L1 cells undifferentiated (left) or differentiated (right) using SCD1 (C12H5) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse lung using SCD1 (C12H5) Rabbit mAb (left) or without primary antibody (right).
Immunohistochemical analysis of paraffin-embedded mouse brown fat using SCD1 (C12H5) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).
Stearoyl-CoA desaturase 1 (SCD1) is a key lipogenic enzyme found in the endoplasmic reticulum that catalyzes the conversion of palmitoyl–CoA and stearoyl–CoA to palmitoleoyl–CoA (16:1) and oleoyl–CoA (18:1) (1-3). Palmitoleate and oleate are the major components of triglycerides, membrane phospholipids and cholesterol esters (1). SCD1-knockout mice show improved insulin sensitivity and reduced body fat (1). Disruption of SCD1 in mouse brown adipose tissue strengthens insulin signaling and results in increased translocation of Glut4 to plasma membrane and enhanced uptake of glucose (4). Furthermore, SCD1 is essential for the onset of diet-induced body weight gain (1) and insulin resistance in the liver (5).
- Ntambi, J.M. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 11482-114866.
- Kato, H. et al. (2006) J. Cell. Sci. 119, 2342-2353.
- Ozols, J. (1997) Mol. Biol. Cell 8, 2281-2290.
- Rahman, S.M. et al. (2005) Am. J Physiol. Endocrinol. Metab. 288, E381-387.
- Gutiérrez-Juárez, R. et al. (2006) J. Clin. Invest. 116, 1686-1695.
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For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5® is a registered trademark of Biostatus Limited.
BODIPY® is a registered trademark of Molecular Probes, Inc.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
This antibody is developed, validated, and produced by CST using in part technology under license (granting certain rights including those under U.S. Patent No. 5,675,063) from Epitomics, Inc.