Cell Signaling Technology

Product Pathways - Glucose Metabolism

SCD1 (C12H5) Rabbit mAb #2794

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP IHC-P IF-IC M Endogenous 37 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  M=Mouse
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

SCD1 (C12H5) Rabbit mAb detects endogenous levels of total SCD1 protein.

Source / Purification

Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from the sequence around Leu35 of mouse SCD1.

Western Blotting

Western Blotting

Western blot analysis of 3T3-L1 cell lysates using SCD1 (C12H5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded 3T3-L1 cells undifferentiated (left) or differentiated (right) using SCD1 (C12H5) Rabbit mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse lung using SCD1 (C12H5) Rabbit mAb (left) or without primary antibody (right).


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded mouse brown fat using SCD1 (C12H5) Rabbit mAb in the presence of control peptide (left) or antigen-specific peptide (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of NIH/3T3-L1 cells, using SCD1 (C12H5) Antibody (red) showing cytoplasmic localization in differentiated cells. Lipid droplets have been labeled with BODIPY 493/503 (green). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

Stearoyl-CoA desaturase 1 (SCD1) is a key lipogenic enzyme found in the endoplasmic reticulum that catalyzes the conversion of palmitoyl?CoA and stearoyl?CoA to palmitoleoyl?CoA (16:1) and oleoyl?CoA (18:1) (1-3). Palmitoleate and oleate are the major components of triglycerides, membrane phospholipids and cholesterol esters (1). SCD1-knockout mice show improved insulin sensitivity and reduced body fat (1). Disruption of SCD1 in mouse brown adipose tissue strengthens insulin signaling and results in increased translocation of Glut4 to plasma membrane and enhanced uptake of glucose (4). Furthermore, SCD1 is essential for the onset of diet-induced body weight gain (1) and insulin resistance in the liver (5).

  1. Ntambi, J.M. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 11482-114866.
  2. Kato, H. et al. (2006) J. Cell. Sci. 119, 2342-2353.
  3. Ozols, J. (1997) Mol. Biol. Cell 8, 2281-2290.
  4. Rahman, S.M. et al. (2005) Am. J Physiol. Endocrinol. Metab. 288, E381-387.
  5. Gutierrez-Juarez, R. et al. (2006) J. Clin. Invest. 116, 1686-1695.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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