Product Pathways - Lymphocyte Signaling
Lyn (C13F9) Rabbit mAb #2796
|W IP IHC-P||H M R Mk||Endogenous||56||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Lyn (C13F9) Rabbit mAb detects endogenous levels of total Lyn protein. This antibody does not cross-react with any other Src-family members.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the amino-terminal sequence of human Lyn.
Western blot analysis of cell extracts from various cell types using Lyn (C13F9) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded non-hodgkins lymphoma using Lyn (C13F9) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human tonsil tissue using Lyn (C13F9) Rabbit mAb in the presence of control peptide (left) and antigen specific peptide (right).
Lyn, one of the Src family members, is predominantly expressed in hematopoietic cells (1). Two tyrosine residues have been reported to play a crucial role in the regulation of protein tyrosine kinases of the Src family. Autophosphorylation of Tyr396 (equivalent to Tyr416 of Src), located in the catalytic domain, correlates with enzyme activation. Csk-mediated phosphorylation of the carboxy-terminal Tyr507 (equivalent to Tyr527 of Src) inactivates the kinase. Tyrosine phosphorylation and activation of Lyn occurs upon association with cell surface receptors such as the B cell Ag receptor (BCR) and CD40 (2-4). Studies using knockout mice have shown that the net effect of Lyn deficiency is to render B cells hypersensitive to BCR stimulation (5-7), suggesting that the most critical role for Lyn in vivo is in the down-regulation of B cell responses. Lyn is also involved in controlling the migration and development of specific B cell populations (8).
- Yamanashi, Y. et al. (1989) Proc. Natl. Acad. Sci. USA 86, 6538-6542.
- Yamanashi, Y. et al. (1991) Science 251, 192-194.
- Burkhardt, A.L. et al. (1991) Proc. Natl. Acad. Sci. USA 88, 7410-7414.
- Ren, C.L. et al. (1994) J. Exp. Med. 179, 673-680.
- Wang, J. et al. (1996) J. Exp. Med. 184, 831-838.
- Chan, V.W. et al. (1997) Immunity 7, 69-81.
- Hibbs, M. L. et al. (1995) Cell 83, 301-311.
- Seo, S.J. et al. (2001) J. Immunol. 166, 3710-3723.
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For Research Use Only. Not For Use In Diagnostic Procedures.