Product Pathways - Glucose Metabolism
Hexokinase I Antibody #2804
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H Mk (B) | Endogenous | 102 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
Mk=Monkey
B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Hexokinase I Antibody detects endogenous levels of total hexokinase I protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to the sequence of human hexokinase I. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using Hexokinase I Antibody.
Background
Hexokinase catalyzes the conversion of glucose to glucose-6-phosphate, the first step in glycolysis. Four distinct mammalian hexokinase isoforms, designated as hexokinase I, II, III and IV (glucokinase), have been identified. Hexokinases I, II and III are associated with the outer mitochondrial membrane and are critical for maintaining an elevated rate of aerobic glycolysis in cancer cells (Warburg Effect) (1) in order to compensate for the increased energy demands associated with the rapid cell growth and proliferation (2,3).
- Warburg, O. (1956) Science 123, 309-314.
- Semenza, G.L. (2000) Crit. Rev. Biochem. Mol. Biol. 35, 71-103.
- Smith, T.A. (2000) Br. J. Biomed. Sci. 57, 170-178.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2106 Hexokinase II Antibody
- 3188 Aldolase Antibody
- 2118 GAPDH (14C10) Rabbit mAb
- 3198 PKM2 Antibody
- 3186 PKM1/2 Antibody
- 9997 Tris Buffered Saline with Tween 20 (TBST - 10X)
- 9998 BSA
- 9999 Nonfat Dry Milk
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
