Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

SH2D1A (XLP 1D12) Rat mAb #2805

Applications Reactivity Sensitivity MW (kDa) Isotype
W F H Endogenous 14 kDa Rat IgG2a

Applications Key:  W=Western Blotting  F=Flow Cytometry
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

SH2D1A (XLP 1D12) Rat mAb detects endogenous levels of total SH2D1A protein.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a recombinant full-length SH2D1A protein.

Western Blotting

Western Blotting

Western blot analysis of total cell lysates from Molt4 and Jurkat cells using SH2D1A (XLP 1D12) Rat mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat cells using SH2D1A (XLP 1D12) Rat mAb (blue) compared to a nonspecific negative control antibody (red).

Background

SH2D1A and SH2D1B are small, adaptor proteins with a single SH2-domain that play important signal transduction roles mediated by the signaling lymphocytic activation molecule (SLAM) family receptors (1). SH2D1A (also called SAP or SLAM-associated protein) is frequently mutated in patients with X-linked lymphoproliferative disease (Duncan’s disease), which is characterized by extreme susceptibility to Epstein-Barr virus; approximately 50 different SH2D1A mutations have been reported to date (2-4). The single SH2D1B gene in humans (also called EAT-2 or Ewing's sarcoma's/FLI1-activated transcript 2) is present as a pair of duplicated EAT-2A and EAT-2B genes with identical genomic organization in mouse and rat (5,6).

  1. Latour, S. and Veillette, A. (2004) Semin. Immunol. 16, 409-419.
  2. Nichols, K.E. et al. (2005) Immunol. Rev. 203, 180-199.
  3. Engel, P. et al. (2003) Nat. Rev. Immunol. 3, 813-821.
  4. Sumegi, J. et al. (2002) Leuk. Lymphoma 43, 1189-1201.
  5. Roncagalli, R. et al. (2005) Nat. Immunol. 6, 1002-1010.
  6. Calpe, S. et al. (2006) Immunogenetics 58, 15-25.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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