Cell Signaling Technology

Product Pathways - MAPK Signaling

Phospho-MLK3 (Thr277/Ser281) Antibody #2811

Applications Reactivity Sensitivity MW (kDa) Source
W H (M) Endogenous 92 to 115 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse
Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-MLK3 (Thr277/Ser281) Antibody detects endogenous levels of MLK3 phosphorylated at Thr277 and Ser281. This antibody does not cross-react with phosphorylated MLK1, MLK2, or other mixed lineage kinases.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr277/Ser281 of human MLK3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from A431 cells, untreated or sorbitol-treated, using Phospho-MLK3 (Thr277/Ser281) Antibody (upper) or MLK3 Antibody #2817 (lower).

Background

Mixed lineage kinase 3 (MLK3) is a serine/threonine kinase that has an amino-terminal SH3 domain followed by the kinase domain and two leucine zippers, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. CRIB triggers the dimerization of MLK3 via its tandem leucine zippers, followed by the intramolecular phosphorylation and subsequent activation of MLK3 (1,2). Autophosphorylation of Thr277 and Ser281 is essential for MLK3 kinase activity (3). Ser281 is also phosphorylated by HPK in an in vitro kinase assay (3). MLK3 functions as a MAPKKK of the SAPK/JNK stress pathway by directly phosphorylating SEK1/MKK4 and MKK7, although it is controversial whether MLK3 is involved in p38 stress pathway activation (1,4). MLK3 also functions as an IκB kinase and mediates the activation of the transcriptional factor NF-κB stimulated by CD3/CD28, suggesting a role for MLK3 in immune and inflammatory responses (5).

  1. Teramoto, H. et al. (1996) J. Biol. Chem. 271, 27225-27228.
  2. Leung, I. W. et al. (1998) J. Biol. Chem. 273, 32408-32415.
  3. Leung, I.W. and Lassam, N. (2001) J. Biol. Chem. 276, 1961-1967.
  4. Tibbles, L. A. et al. (1996) EMBO J. 15, 7026-7035.
  5. Hehner, S. P. et al. (2000) Mol. Cell. Biol. 20, 2556-2568.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

Product Pathways

Drug Discovery Tools

Featured Technologies

Protein Classes