Cell Signaling Technology

Product Pathways - Cell Cycle / Checkpoint

RecQ4 Antibody #2814

Applications Reactivity Sensitivity MW (kDa) Source
W H R (Mk) Endogenous 150 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

RecQ4 Antibody detects endogenous levels of total RecQ4 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the amino terminus of human RecQ4. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts of HeLa, A431 and H3255 cells using RecQ4 Antibody.

Background

RECQL4 is a member of the RecQ family of DNA helicases that plays an important role in global genomic stability. There are five members of this family in humans, and mutations in three of these, BLM, WRN and RECQL4, give rise to disorders that are characterized by premature aging and a predisposition to cancer (1). Despite the presence of a helicase domain, no helicase activity has been reported for RECQL4. Rather, RECQL4 has an ATPase function that is stimulated by ssDNA, and a ssDNA annealing activity that is inhibited by RPA (2). RECQL4 has been reported to interact with ubiquitin ligases UBR1 and UBR2 (3). The role of RECQL4 in tumor suppression and the maintenance of genomic integrity has been attributed to it’s activities associated with the regulation of DNA replication, and DNA recombination and repair (4-6).Mutations in the RECQL4 gene have been identified in a subset of patients with Rothmund-Thomson syndrome (RTS) - a disorder characterized by growth deficiency, skin and skeletal abnormalities, and cancer predisposition. Two more autosomal recessive disorders have been associated with RECQL4 gene mutations: RAPADILINO, and Baller-Gerold syndromes (4).

  1. Hanada, K. and Hickson, I.D. (2007) Cell Mol Life Sci.
  2. Macris, M.A. et al. (2006) DNA Repair (Amst) 5, 172-80.
  3. Yin, J. et al. (2004) Hum Mol Genet 13, 2421-30.
  4. Dietschy, T. et al. (2007) Cell Mol Life Sci 64, 796-802.
  5. LeRoy, G. et al. (2005) Nucleic Acids Res 33, 6251-7.
  6. Sengupta, S. et al. (2005) Oncogene 24, 1738-48.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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