Product Pathways - Translational Control
Grp75 Antibody #2816
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IF-IC | H M R Mk | Endogenous | 75 | Rabbit |
Applications Key:
W=Western Blotting
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Grp75 Antibody detects endogenous levels of total Grp75 protein.
Source / Purification
Polyclonal antibodies are produced by immunizing rabbits with a synthetic peptide (KLH-coupled) derived from a sequence of human Grp75. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts from various cell types using Grp75 Antibody.
IF-IC
Confocal immunofluorescent analysis of MCF-7 cells using Grp75 Antibody (green) showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat-inducible (1,2). This protein is essential for transporting many mitochondrial proteins from the cytoplasm to mitochondria (3). Grp75 inactivates the tumor suppressor p53 (4). Studies found that Grp75 is overexpressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (5). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (6).
- Kaul, S.C. et al. (1993) Biochem Biophys Res Commun 193, 348-55.
- Wadhwa, R. et al. (1993) J Biol Chem 268, 6615-21.
- Schneider, H.C. et al. (1994) Nature 371, 768-74.
- Wadhwa, R. et al. (1998) J Biol Chem 273, 29586-91.
- Wadhwa, R. et al. (2006) Int J Cancer 118, 2973-80.
- Kaul, S.C. et al. (2003) Exp Cell Res 286, 96-101.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.
