Cell Signaling Technology

Product Pathways - Translational Control

Grp75 Antibody #2816

Applications Reactivity Sensitivity MW (kDa) Source
W IF-IC H M R Mk Endogenous 75 Rabbit

Applications Key:  W=Western Blotting  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Grp75 Antibody detects endogenous levels of total Grp75 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to a sequence of human Grp75. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Grp75 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of MCF-7 cells using Grp75 Antibody (green) showing colocalization with mitochondria that have been labeled with MitoTracker® Red CMXRos (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).

Background

Grp75, also known as mortalin, is a member of Hsp70 family of chaperone proteins that is not heat-inducible (1,2). This protein is essential for transporting many mitochondrial proteins from the cytoplasm to mitochondria (3). Grp75 inactivates the tumor suppressor p53 (4). Studies found that Grp75 is overexpressed in many tumor tissues and immortalized human cell lines, suggesting its role in the tumor formation (5). Grp75 is also implicated in cell aging, as its overexpression appears to prolong the life span of human fibroblasts (6).

  1. Kaul, S.C. et al. (1993) Biochem Biophys Res Commun 193, 348-55.
  2. Wadhwa, R. et al. (1993) J Biol Chem 268, 6615-21.
  3. Schneider, H.C. et al. (1994) Nature 371, 768-74.
  4. Wadhwa, R. et al. (1998) J Biol Chem 273, 29586-91.
  5. Wadhwa, R. et al. (2006) Int J Cancer 118, 2973-80.
  6. Kaul, S.C. et al. (2003) Exp Cell Res 286, 96-101.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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