Cell Signaling Technology

Product Pathways - MAPK Signaling

MLK3 Antibody #2817

Applications Reactivity Sensitivity MW (kDa) Source
W H M R Hm Mk Endogenous 92 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  Hm=Hamster  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MLK3 Antibody detects endogenous levels of total MLK3 protein. This antibody does not cross-react with MLK1, MLK2 and other mixed lineage kinases.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human MLK3. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa, NIH/3T3, C6 and COS cells using MLK3 Antibody.

Background

Mixed lineage kinase 3 (MLK3) is a serine/threonine kinase that has an amino-terminal SH3 domain followed by the kinase domain and two leucine zippers, a cdc42/Rac1 binding (CRIB) domain and several other domains/motifs at the carboxy-terminal region. CRIB triggers the dimerization of MLK3 via its tandem leucine zippers, followed by the intramolecular phosphorylation and subsequent activation of MLK3 (1,2). Autophosphorylation of Thr277 and Ser281 is essential for MLK3 kinase activity (3). Ser281 is also phosphorylated by HPK in an in vitro kinase assay (3). MLK3 functions as a MAPKKK of the SAPK/JNK stress pathway by directly phosphorylating SEK1/MKK4 and MKK7, although it is controversial whether MLK3 is involved in p38 stress pathway activation (1,4). MLK3 also functions as an IκB kinase and mediates the activation of the transcriptional factor NF-κB stimulated by CD3/CD28, suggesting a role for MLK3 in immune and inflammatory responses (5).

  1. Teramoto, H. et al. (1996) J. Biol. Chem. 271, 27225-27228.
  2. Leung, I. W. et al. (1998) J. Biol. Chem. 273, 32408-32415.
  3. Leung, I.W. and Lassam, N. (2001) J. Biol. Chem. 276, 1961-1967.
  4. Tibbles, L. A. et al. (1996) EMBO J. 15, 7026-7035.
  5. Hehner, S. P. et al. (2000) Mol. Cell. Biol. 20, 2556-2568.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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