Product Pathways - Chromatin Regulation / Epigenetics
Ring1A Antibody #2820
PhosphoSitePlus® protein, site, and accession data: RING1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H M R Mk | Endogenous | 58 | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
R=Rat
Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2820:
- Western Blotting
Specificity / Sensitivity
Ring1A Antibody detects endogenous levels of total Ring1A protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to human Ring1A. Antibodies are purified by protein A and peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts of MOLT4 annd LNCaP cells using Ring1A Antibody.
Background
Ring1A plays a role in polycomb group (PcG) protein function. PcG proteins are critically involved in transcriptional repression of Hox genes during development (1,2). PcG proteins form two distinct complexes: EED-EZH2 and the PRC complex, which is composed of at least Bmi1 and Ring1A/Ring1B. The EZH2-containing complex is responsible for the methylation of H3K27, and the PRC complex ubiquitylates H2A. EZH2 methylation is required prior to PRC ubiquitylation, and both are essential for Hox gene repression (3). It has recently been shown that PcG proteins silence a group of developmentally important regulator genes, referred to as bivalent genes (4). This regulation may be responsible for the ability of stem cells to self renew or switch to differentiate into multipotent progenitors. Aberrant epigenetic silencing by PcG proteins is also thought to be important in tumorigenesis (5).
- Cao, R. et al. (2005) Mol Cell 20, 845-54.
- Otte, A.P. and Kwaks, T.H. (2003) Curr Opin Genet Dev 13, 448-54.
- Cao, R. et al. (2005) Mol Cell 20, 845-54.
- Stock, J.K. et al. (2007) Nat Cell Biol 9, 1428-35.
- Sparmann, A. and van Lohuizen, M. (2006) Nat Rev Cancer 6, 846-56.
Application References
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
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For Research Use Only. Not For Use In Diagnostic Procedures.
