Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

Phospho-PLCγ1 (Tyr783) Antibody #2821

Applications Reactivity Sensitivity MW (kDa) Source
W IP F H M R Endogenous 155 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  F=Flow Cytometry
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by Western blot.

Protocols

Specificity / Sensitivity

Phospho-PLCγ1 (Tyr783) Antibody detects PLCγ1 only when phosphorylated at tyrosine 783. It does not cross-react with phosphorylated PLCγ2 or other PLCs.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phosphopeptide (KLH-coupled) corresponding to residues surrounding tyrosine 783 of human PLCγ1. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from NIH/3T3 cells, untreated or PDGF-stimulated for the indicated times, using Phospho-PLCγ1 (Tyr783) Antibody (upper) or PLCγ1 Antibody #2822 (lower).

IP

IP

Immunoprecipitation of PLCγ1 from untreated or PDGF treated NIH/3T3 cells, using Phospho-PLCγ1 (Tyr783) Antibody followed by Western blot analysis with the same antibody.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of NIH3T3 cells, untreated (blue) or PDGF treated (green), using Phospho-PLCγ1 (Tyr783) Antibody.


Background

Phosphoinositide-specific phospholipase C (PLC) plays a significant role in transmembrane signaling. In response to extracellular stimuli such as hormones, growth factors and neurotransmitters, PLC hydrolyzes phosphatidylinositol 4,5-bisphosphate (PIP2) to generate two secondary messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG) (1). At least four families of PLCs have been identified: PLCβ, PLCγ, PLCδ and PLCε. The PLCβ subfamily includes four members, PLCβ1-4. All four members of the subfamily are activated by α- or β-γ-subunits of the heterotrimeric G-proteins (2,3).Phosphorylation is one of the key mechanisms that regulates the activity of PLC. Phosphorylation of Ser1105 by PKA or PKC inhibits PLCβ3 activity (4,5). Ser537 of PLCβ3 is phosphorylated by CaMKII, and this phosphorylation may contribute to the basal activity of PLCβ3. PLCγ is activated by both receptor and nonreceptor tyrosine kinases (6).PLCγ forms a complex with EGF and PDGF receptors, which leads to the phosphorylation of PLCγ at Tyr771, 783 and 1245 (7). Phosphorylation by Syk at Tyr783 activates the enzymatic activity of PLCγ1 (8).

  1. Singer, W. D. et al. (1997) Annu. Rev. Biochem. 66, 475-509.
  2. Smrcka, A. V. et al. (1991) Science 251, 804-807.
  3. Taylor, S. J. et al. (1991) Nature 350, 516-518.
  4. Yue, C. et al. (1998) J. Biol. Chem. 273, 18023-18027.
  5. Yue, C. et al. (2000) J. Biol. Chem. 275, 30220-30225.
  6. Margolis, B. et al. (1989) Cell 57, 1101-1107.
  7. Kim, H. K. et al. (1991) Cell 65, 435-441.
  8. Wang, Z. et al. (1998) Mol. Cell. Biol. 18, 590-597.

Application References

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Companion Products

This product is for in vitro research use only and is not intended for use in humans or animals. This product is not intended for use as therapeutic or in diagnostic procedures.

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