Cell Signaling Technology

Product Pathways - Chromatin Regulation / Epigenetics

MEP50 Antibody #2823

Applications Reactivity Sensitivity MW (kDa) Source
W IP IF-IC H Endogenous 42 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

MEP50 Antibody detects endogenous levels of total MEP50 protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids surrounding Ser225 of human MEP50. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from U2OS and HeLa cells using MEP50 Antibody.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using MEP50 Antibody (green). Actin filaments have been labeled with DY554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).

Background

MEP50 (methylosome protein 50) is a component of the methylosome, a protein arginine methyltransferase complex that modifies specific arginine residues found in arginine- and glycine-rich regions of some spliceosomal Sm proteins. MEP50 is important for methylosome activity and may regulate the transfer of Sm proteins to the SMN (survival of motor neurons) complex, an early step in the assembly of U snRNPs. Both the methylosome and the SMN complex are essential for the assembly of spliceosomal snRNPs (1).MEP50 is a WD repeat protein that may provide an interface for multiple protein interactions between methylosome proteins. (1). It binds to JBP1, an arginine protein methyltransferase component of the methylosome. MEP50 has been shown to interact with CTD phosphatase FCP1 (CTDP1), a protein that may link the processes of transcriptional elongation and splicing (2), and with SUZ12, a polycomb group protein involved in transcriptional repression (3). JBP1 and MEP50 have also been reported to interact with the methyl-CpG binding protein complex MBD2/NuRD (4).

  1. Friesen, W.J. et al. (2002) J Biol Chem 277, 8243-7.
  2. Licciardo, P. et al. (2003) Nucleic Acids Res 31, 999-1005.
  3. Furuno, K. et al. (2006) Biochem Biophys Res Commun 345, 1051-8.
  4. Le Guezennec, X. et al. (2006) Mol Cell Biol 26, 843-51.

Application References

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Companion Products

This product is intended for research purposes only. The product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

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