Cell Signaling Technology

Product Pathways - Ca / cAMP / Lipid Signaling

cPLA2 Antibody #2832

Applications Reactivity Sensitivity MW (kDa) Source
W IP H M R Mk Endogenous 95 Rabbit

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

cPLA2 Antibody detects endogenous levels of total cPLA2 protein. This antibody does not cross-react with the secreted PLA2 (sPLA2) or cytosolic Ca2+ independent PLA2 (iPLA2).

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the carboxy-terminal residues of human cPLA2. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HeLa cells, untreated or TNF-α-treated (10 ng/ml for 15 minutes), and NIH/3T3 and COS cells, using Phospho-cPLA2 (Ser505) Antibody #2831 (upper) or cPLA2 Antibody (lower).

IP

IP

Immunoprecipitation of cPLA2 from untreated and TNF-alpha treated HeLa cells followed by Western blot analysis, using cPLA2 Antibody.

Background

Cytosolic phospholipase A2 (cPLA2) is a ubiquitously distributed enzyme that catalyzes the hydrolysis of the sn-2 acyl bond of glycerolipids to produce lysophospholipids and release arachidonic acid (1). cPLA2 has been implicated in diverse cellular responses such as mitogenesis, differentiation, inflammation and cytotoxicity (1). Calcium binding to the amino-terminal CalB domain of cPLA2 promotes the translocation of cPLA2 from cytosol to membrane, where cPLA2 cleaves arachidonic acid from natural membrane (2). Phosphorylation of cPLA2 by MAPK (p42/44 and p38) at Ser505 (3,4) and Ser727 (5) stimulates its catalytic activity.

  1. Leslie, C.C. (1997) J. Biol. Chem. 272, 16709-16712.
  2. Clark, J. D. et al. (1991) Cell 65, 1043-1051.
  3. Lin, L. L. et al. (1993) Cell 72, 269-278.
  4. Kramer, R.M. (1996) J. Biol. Chem. 271, 27723-27729.
  5. Hefner, Y. et al. (2000) J. Biol. Chem. 275, 37542-37551.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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