Product Pathways - Neuroscience
Neurofilament-L (DA2) Mouse mAb #2835
|W IHC-P IF-F||H M R||Endogenous||70||Mouse IgG1|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Neurofilament-L (DA2) Mouse mAb detects endogenous levels of total Neurofilament-L protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with purified and enzymatically dephosphorylated pig neurofilament, light chain.
Western blot analysis of extracts from rat brain, mouse brain, U87-MG cells, C6 cells and human cerebellum, using Neurofilament-L (DA2) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human brain, showing neuronal staining, using Neurofilament-L (DA2) Mouse mAb.
Immunohistochemical analysis of paraffin-embedded human medulloblastoma, using Neurofilament-L (DA2) Mouse mAb.
The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).
- Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-355.
- Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339.
- Hisanaga, S. et al. (1994) Mol. Biol. Cell 5, 161-172.
- Sihag, R.K. et al. (1999) J. Neurochem. 72, 491-499.
- Evans, J. et al. (2001) Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem. J. Neurophysiol. 87, 1076-1085. Applications: IC-IF
- Haddad, L.A. et al. (2002) The TSC1 tumor suppressor hamartin interacts with neurofilament-L and possibly functions as a novel integrator of the neuronal cytoskeleton. J. Biol. Chem. 277, 44180-44186. Applications: IC-IF Western Blotting
- Shaw, G. et al. (2002) Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. 16, 869-871. Applications: IC-IF
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For Research Use Only. Not For Use In Diagnostic Procedures.