Product Pathways - Neuroscience
Neurofilament-L (DA2) Mouse mAb #2835
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IHC-P IF-F | H M R | 70 | Mouse | IgG1 |
Applications Key:
W=Western Blotting
IHC-P=Immunohistochemistry (Paraffin)
IF-F=Immunofluorescence (Frozen)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Neurofilament-L (DA2) Mouse mAb detects endogenous levels of total Neurofilament-L protein.
Source / Purification
Monoclonal antibody is produced by immunizing mice with purified and enzymatically dephosphorylated pig neurofilament, light chain.
Western Blotting
Western blot analysis of extracts from rat brain, mouse brain, U87-MG cells, C6 cells and human cerebellum, using Neurofilament-L (DA2) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human brain, showing neuronal staining, using Neurofilament-L (DA2) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human medulloblastoma, using Neurofilament-L (DA2) Mouse mAb.
IF-F
Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-L (DA2) Mouse mAb (green) and CREB (48H2) Rabbit mAb #9197 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Application References
- Evans, J. et al. (2001) Characterization of mitotic neurons derived from adult rat hypothalamus and brain stem. J. Neurophysiol. 87, 1076-1085. This article references the use of Neurofilament-L (DA2) Mouse mAb in the following applications: IC-IF
- Haddad, L.A. et al. (2002) The TSC1 tumor suppressor hamartin interacts with neurofilament-L and possibly functions as a novel integrator of the neuronal cytoskeleton. J. Biol. Chem. 277, 44180-44186. This article references the use of Neurofilament-L (DA2) Mouse mAb in the following applications: IC-IF Western Blotting
- Shaw, G. et al. (2002) Preferential transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. 16, 869-871. This article references the use of Neurofilament-L (DA2) Mouse mAb in the following applications: IC-IF
Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!
Companion Products
- 2148 α/β-Tubulin Antibody
- 4906 Hamartin/TSC1 Antibody
- 7072 Phototope®-HRP Western Blot Detection System, Anti-mouse IgG, HRP-linked Antibody
- 7076 Anti-mouse IgG, HRP-linked Antibody
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
- 4781 Phospho-PKA C (Thr197) Antibody
- 4782 PKA C-α Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
