Cell Signaling Technology

Product Pathways - Neuroscience

Neurofilament-H (RMdO 20) Mouse mAb #2836

Applications Reactivity MW (kDa) Source Isotype
W IP IHC-P IF-F H M R 180-220 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-F=Immunofluorescence (Frozen)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Neurofilament-H (RMdO 20) Mouse mAb detects endogenous levels of total Neurofilament-H protein. Neurofilament-H (RMdO 20) Mouse mAb has been reported to detect NFM and NFH in human samples but only NFH in mouse, rat or bovine samples (Lee, V.M. et al., 1988).

Source / Purification

Monoclonal antibody is produced by immunizing mice with rat neurofilament.

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse brain, rat brain or CAD cells, using Neurofilament-H (RMdO 20) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human brain, using Neurofilament-H (RMdO 20) Mouse mAb.

IF-F

IF-F

Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-H (RMdO 20) Mouse mAb (green) and Calbindin Antibody #2136 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


Background

Studies of NFH (-/-) mice suggest that NFH modulates ion channel functions in large myelinated fibers (5).

  1. Kriz, J. et al. (2000) Brain Res 885, 32-44.

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