Product Pathways - Neuroscience
Neurofilament-L (C28E10) Rabbit mAb #2837
|W IHC-P IF-F||H M R||Endogenous||70||Rabbit IgG|
Reactivity Key: H=Human M=Mouse R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Specificity / Sensitivity
Neurofilament-L (C28E10) Rabbit mAb detects endogenous levels of total Neurofilament-L protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide surrounding Glu450 of human Neurofilament-L.
Western blot analysis of extracts from mouse brain, HeLa cells and rat brain, using Neurofilament-L (C28E10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded mouse brain using Neurofilament-L (C28E10) Rabbit mAb.
Immunohistochemical analysis of paraffin-embedded human brain using Neurofilament-L (C28E10) Rabbit mAb in the presence of control peptide (left) or Neurofilament-L blocking peptide #1005 (right).
The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).
- Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-355.
- Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339.
- Hisanaga, S. et al. (1994) Mol. Biol. Cell 5, 161-172.
- Sihag, R.K. et al. (1999) J. Neurochem. 72, 491-499.
- Mullenbrock, S. et al. (2011) J Biol Chem 286, 45131-45. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.