Product Pathways - Neuroscience
Neurofilament-M (RMO 14.9) Mouse mAb #2838
PhosphoSitePlus® protein, site, and accession data: NFM
| Applications | Reactivity | Sensitivity | MW (kDa) | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC | H M R | Endogenous | 160 | Mouse IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
Neurofilament-M (RMO 14.9) Mouse mAb detects endogenous levels of total Neurofilament-M protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with rat neurofilament, medium chain.
Western Blotting
Western blot analysis of extracts from rat brain, untreated or treated with lambda phosphatase, using Neurofilament-M (RMO 14.9) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human brain, using Neurofilament-M (RMO 14.9) Mouse mAb.
IF-F
Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-M (RMO 14.9) Mouse mAb (green) and Calbindin Antibody #2136 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Background
The cytoskeleton consists of three types of cytosolic fibers: actin microfilaments, intermediate filaments, and microtubules. Neurofilaments are the major intermediate filaments found in neurons and consist of light (NFL), medium (NFM), and heavy (NFH) subunits (1). Similar in structure to other intermediate filament proteins, neurofilaments have a globular amino-terminal head, a central α-helical rod domain, and a carboxy-terminal tail. A heterotetrameric unit (NFL-NFM and NFL-NFH) forms a protofilament, with eight protofilaments comprising the typical 10 nm intermediate filament (2). While neurofilaments are critical for radial axon growth and determine axon caliber, microtubules are involved in axon elongation. PKA phosphorylates the head domain of NFL and NFM to inhibit neurofilament assembly (3,4). Research studies have shown neurofilament accumulations in many human neurological disorders including Parkinson's disease (in Lewy bodies along with α-synuclein), Alzheimer's disease, Charcot-Marie-Tooth disease, and Amyotrophic Lateral Sclerosis (ALS) (1).
- Al-Chalabi, A. and Miller, C.C. (2003) Bioessays 25, 346-355.
- Cohlberg, J.A. et al. (1995) J. Biol. Chem. 270, 9334-9339.
- Hisanaga, S. et al. (1994) Mol. Biol. Cell 5, 161-172.
- Sihag, R.K. et al. (1999) J. Neurochem. 72, 491-499.
Application References
- Büki, A. et al. (2000) J. Histochem. Cytochem. 48, 153-61. Applications: IHC-P (paraffin)
- Gabanella, F. et al. (2005) Hum. Mol. Genet. 14, 3629-42. Applications: Western Blotting
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For Research Use Only. Not For Use In Diagnostic Procedures.
