Product Pathways - Neuroscience
Neurofilament-M (RMO 14.9) Mouse mAb #2838
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP IHC-P IF-IC | H M R | 160 | Mouse | IgG1 |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
IHC-P=Immunohistochemistry (Paraffin)
IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:
H=Human
M=Mouse
R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Neurofilament-M (RMO 14.9) Mouse mAb detects endogenous levels of total Neurofilament-M protein.
Source / Purification
Monoclonal antibody is produced by immunizing mice with rat neurofilament, medium chain.
Western Blotting
Western blot analysis of extracts from rat brain, untreated or treated with lambda phosphatase, using Neurofilament-M (RMO 14.9) Mouse mAb.
IHC-P (paraffin)
Immunohistochemical analysis of paraffin-embedded human brain, using Neurofilament-M (RMO 14.9) Mouse mAb.
IF-F
Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-M (RMO 14.9) Mouse mAb (green) and Calbindin Antibody #2136 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).
Application References
- Buki, A. et al. (2000) J. Histochem. Cytochem. 48, 153-61. This article references the use of Neurofilament-M (RMO 14.9) Mouse mAb in the following applications: IHC-P (paraffin)
- Gabanella, F. et al. (2005) Hum. Mol. Genet. 14, 3629-42. This article references the use of Neurofilament-M (RMO 14.9) Mouse mAb in the following applications: Western Blotting
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