Cell Signaling Technology

Product Pathways - Neuroscience

Neurofilament-M (RMO 14.9) Mouse mAb #2838

Applications Reactivity MW (kDa) Source Isotype
W IP IHC-P IF-IC H M R 160 Mouse IgG1

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Neurofilament-M (RMO 14.9) Mouse mAb detects endogenous levels of total Neurofilament-M protein.

Source / Purification

Monoclonal antibody is produced by immunizing mice with rat neurofilament, medium chain.

Western Blotting

Western Blotting

Western blot analysis of extracts from rat brain, untreated or treated with lambda phosphatase, using Neurofilament-M (RMO 14.9) Mouse mAb.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human brain, using Neurofilament-M (RMO 14.9) Mouse mAb.

IF-F

IF-F

Confocal immunofluorescent image of mouse cerebellum labeled with Neurofilament-M (RMO 14.9) Mouse mAb (green) and Calbindin Antibody #2136 (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


Application References

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