Cell Signaling Technology

Product Pathways - Lymphocyte Signaling

SHIP2 (C76A7) Rabbit mAb #2839

Applications Reactivity MW (kDa) Source Isotype
W IP IF-IC F H 160 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation  IF-IC=Immunofluorescence (Immunocytochemistry)  F=Flow Cytometry
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

SHIP2 (C76A7) Rabbit mAb detects endogenous levels of total SHIP2 protein.

Source / Purification

Monoclonal antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues surrounding Ala1083 of human SHIP2.

Western Blotting

Western Blotting

Western blot analysis of extracts from OVCAR8, LN18 and HeLa cells using SHIP2 (C76A7) Rabbit mAb.

Flow Cytometry

Flow Cytometry

Flow cytometric analysis of Jurkat (blue) and HeLa cells (green) using SHIP2 (C76A7) Rabbit mAb.

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa (left) and Jurkat cells (right) using SHIP2 (C76A7) Rabbit mAb (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).


Background

SH2-containing inositol phosphatase 1 (SHIP1) is a hematopoietic phosphatase that hydrolyzes phosphatidylinositol-3,4,5-triphosphate to phosphatidylinositol-3,4-bisphosphate (1). SHIP1 is a cytosolic phosphatase with an SH2 domain in its amino terminus and two NPXY Shc binding motifs in its carboxy terminus (1,2). Upon receptor cross-linking, SHIP is first recruited to the membrane junction through binding of its SH2 domain to the phospho-tyrosine in the ITIM motif (2), followed by tyrosine phosphorylation on the NPXY motif (2). The membrane relocalization and phosphorylation on the NPXY motif is essential for the regulatory function of SHIP1 (3-5). Its effect on calcium flux, cell survival, growth, cell cycle arrest and apoptosis is mediated through the PI3K and Akt pathways (3-5). Tyrosine 1021 is located in one of the NPXY motifs in SHIP1, and its phosphorylation is important for SHIP1 function (6).

SHIP2, a homolog of SHIP1, is highly expressed in heart, skeletal muscle and placenta (7). SHIP2 negatively regulates insulin signaling (8) and polymorphisms in SHIP2 have been linked to hyperglycemia (9). Recent studies also suggest SHIP2 as a therapeutic target for the treatment of both obesity and type 2 diabetes (10,11).

  1. Tridandapani, S. et al. (1997) Mol Cell Biol 17, 4305-11.
  2. Liu, L. et al. (1997) J Biol Chem 272, 8983-8.
  3. Malbec, O. et al. (2001) J Biol Chem 276, 30381-91.
  4. Carver, D.J. et al. (2000) Blood 96, 1449-56.
  5. Scharenberg, A.M. et al. (1998) EMBO J 17, 1961-72.
  6. Sattler, M. et al. (2001) J Biol Chem 276, 2451-8.
  7. Pesesse, X. et al. (1997) Biochem Biophys Res Commun 239, 697-700.
  8. Wada, T. et al. (2001) Mol Cell Biol 21, 1633-46.
  9. Ishida, S. et al. (2006) Pancreas 33, 63-7.
  10. Dyson, J.M. et al. (2005) Int J Biochem Cell Biol 37, 2260-5.
  11. Sasaoka, T. et al. (2006) Pharmacol Ther 112, 799-809.

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