Cell Signaling Technology

Product Pathways - Glucose Metabolism

Phospho-C/EBPalpha (Ser21) Antibody #2841

Applications Reactivity MW (kDa) Source
W H M (R) (B) 45 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  M=Mouse  R=Rat  B=Bovine
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-C/EBPalpha (Ser21) Antibody detects endogenous levels of C/EBPalpha only when phosphorylated at serine 21. This antibody does not cross-react with other phosphorylated C/EBP isoforms.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser21 of human C/EBPalpha. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts of COS cells untransfected (lane 1), or transfected with wild-type mouse C/EBPalpha (lane 2), S21A (lane 3), and S21D (lane 4) mutants, using Phospho-C/EBPalpha (Ser21) Antibody (upper) or C/EBPalpha Antibody (lower). (Provided by Dr. Hanna Radomska, Beth Israel Deaconess Medical Center, Boston, MA).

Western Blotting

Western Blotting

Western blot analysis of extracts from mouse adipocytes treated with insulin for the indicated times, using Phospho-C/EBPalpha (Ser21) Antibody.

Background

CCAAT/enhancer-binding proteins (C/EBPs) are a family of transcription factors that are critical for cellular differentiation, terminal function and inflammatory response (1). Six members of the family have been characterized (C/EBPα, β, δ, γ, ε and ζ) and are distributed in a variety of tissues (1). Translation from alternative start codons results in two isoforms of C/EBPα (p42 and p30), which are both strong transcriptional activators (2). It has been reported that insulin and insulin-like growth factor-1 stimulate the dephosphorylation of C/EBPα, which may play a key role in insulin induced repression of GLUT4 transcription (3). Phosphorylation of C/EBPα at Thr222, Thr226 and Ser230 by GSK3 seems to be required for adipogenesis (4).

  1. Lekstrom-Hims, J. and Xanthopoulos, K.G. (1998) J. Biol. Chem. 273, 28545-28548.
  2. Lin, F. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 9606-9610.
  3. Hemati, N. et al. (1997) J. Biol. Chem. 272, 25913-25919.
  4. Ross, S.E. et al. (1999) Mol. Cell. Biol. 19, 8433-8441.
  5. Radomska, H.S. et al. (2006) J Exp Med 203, 371-81.

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