Cell Signaling Technology

Product Pathways - Cytoskeletal Signaling

Vav2 (C64H2) Rabbit mAb #2848

Applications Reactivity Sensitivity MW (kDa) Isotype
W IP H Mk Endogenous 100 Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Vav2 (C64H2) Rabbit mAb detects endogenous levels of total Vav2 protein. The antibody is not expected to cross-react with Vav1 or Vav3 based on the sequence of the immunogenic peptide.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to residues near the carboxy terminus of human Vav2.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Vav2 (C64H2) Rabbit mAb.

Background

Vav proteins belong to the Dbl family of guanine nucleotide exchange factors (GEFs) for Rho/Rac small GTPases. The three identified mammalian Vav proteins (Vav1, Vav2 and Vav3) differ in their expression. Vav1 is expressed only in hematopoietic cells and is involved in the formation of the immune synapse. Vav2 and Vav3 are more ubiquitously expressed. Vav proteins contain the Dbl homology domain, which confers GEF activity, as well as protein interaction domains that allow them to function in pathways regulating actin cytoskeleton organization (reviewed in 1). Phosphorylation stimulates the GEF activity of Vav protein towards Rho/Rac (2,3).

  1. Hornstein, I. et al. (2004) Cell. Signal. 16, 1-11.
  2. Crespo, P. et al. (1997) Nature 385, 169-172.
  3. Han, J. et al. (1997) Mol. Cell. Biol. 17, 1346-1353.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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