Product Pathways - Development
β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 488 Conjugate) #2849
|2849S||100 µl (50 tests)||---||In Stock||---|
|2849||carrier free and custom formulation / quantity||email request|
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|IF-F||1:50||Human, Rat||Endogenous||Mouse IgG1|
Species cross-reactivity is determined by western blot using the unconjugated antibody.
Applications Key: IF-F=Immunofluorescence (Frozen), IF-IC=Immunofluorescence (Immunocytochemistry), F=Flow Cytometry
Species predicted to react based on 100% sequence homology: Mouse, Monkey, Pig.
Specificity / Sensitivity
β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 488 Conjugate) detects endogenous levels of total β-catenin protein.
Source / Purification
Monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the carboxy terminus of human β-catenin. The antibody was conjugated to Alexa Fluor® 488 under optimal conditions with an F/P ratio of 2-5.
Flow cytometric analysis of NCI-H28 cells (blue) and HeLa cells (green) using β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 488 Conjugate).
Confocal immunofluorescent analysis of HeLa cells using β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 488 Conjugate (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
Confocal immunofluorescent analysis of rat heart using β-Catenin (L54E2) Mouse mAb (Alexa Fluor® 488 Conjugate (green). and Desmin (D93F5) XP® Rabbit mAb (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).
This Cell Signaling Technology antibody is conjugated to Alexa Fluor® 488 fluorescent dye and tested in-house for direct flow cytometry and immunofluorescent analysis in human cells. The antibody is expected to exhibit the same species cross-reactivity as the unconjugated β-Catenin (L54E2) Mouse mAb (IF Preferred) #2677.
β-Catenin is a key downstream effector in the Wnt signaling pathway (1). It is implicated in two major biological processes in vertebrates: early embryonic development (2) and tumorigenesis (3). CK1 phosphorylates β-catenin at Ser45. This phosphorylation event primes β-catenin for subsequent phosphorylation by GSK-3β (4-6). GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41 (7). Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines (8).
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- Wodarz, A. and Nusse, R. (1998) Annu. Rev. Cell. Dev. Biol. 14, 59-88.
- Polakis, P. (1999) Curr. Opin. Genet. Dev. 9, 15-21.
- Amit, S. et al. (2002) Genes Dev. 16, 1066-1076.
- Lin, C. et al. (2002) Cell 108, 837-847.
- Yanagawa, S. et al. (2002) EMBO J. 21, 1733-1742.
- Yost, C. et al. (1996) Genes Dev. 10, 1443-1454.
- Morin, P.J. (1997) Science 275, 1787-1790.
- Mirlashari, M.R. et al. (2012) Leuk Res 36, 499-508. Applications: IF-IC (In Cells).
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For Research Use Only. Not For Use In Diagnostic Procedures.
DRAQ5® is a registered trademark of Biostatus Limited.
Cell Signaling Technology® is a trademark of Cell Signaling Technology, Inc.
The Alexa Fluor® dye antibody conjugates in this product are sold under license from Molecular Probes, Inc., for research use only, except for use in combination with DNA microarrays. The Alexa Fluor® dyes (except for Alexa Fluor® 430 dye) are covered by pending and issued patents. Alexa Fluor® is a registered trademark of Molecular Probes, Inc.