Product Pathways - DNA Damage
XLF Antibody #2854
PhosphoSitePlus® protein, site, and accession data: NHEJ1
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W IP | H | Endogenous | 39 | Rabbit |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
Specificity / Sensitivity
XLF Antibody detects endogenous levels of total XLF protein.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to amino acids near the carboxy terminus of human XLF. Antibodies are purified by peptide affinity chromatography.
Western Blotting
Western blot analysis of extracts of various cell lines using XLF Antibody.
Background
Double stranded DNA breaks (DSB’s) are the most toxic of DNA lesions. They occur in response to genotoxic stress, and they are also an obligate intermediate in the V(D)J recombination events in the immune system. The mechanism by which cells deal with DSB’s is known as NHEJ (non-homologous end-joining), and involves a core group of proteins that includes Ku, DNA-PK, XRCC4, and XLF (1). XLF, also known as Cernunnos, was originally discovered as a mutated protein from cells of individuals who displayed features of growth retardation, microcephaly, and immunodeficiency (2). These cells were sensitive to ionizing radiation and defective in V(D)J recombination. Exogenous expression of wild type XLF corrected these deficiencies (3), indicating that XLF is a critical component of the NHEJ response. XLF physically interacts with and may stimulate the ligase activity of XRCC4 (3).
- Tsai, C.J. et al. (2007) Proc Natl Acad Sci USA 104, 7851-6.
- Buck, D. et al. (2006) Cell 124, 287-99.
- Ahnesorg, P. et al. (2006) Cell 124, 301-13.
Application References
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For Research Use Only. Not For Use In Diagnostic Procedures.
