Product Pathways - Phosphatases
Phospho-MKP1 (Ser359) (125E2) Rabbit mAb #2857
| Applications | Reactivity | MW (kDa) | Source | Isotype |
|---|---|---|---|---|
| W IP | H | 40 | Rabbit | IgG |
Applications Key:
W=Western Blotting
IP=Immunoprecipitation
Reactivity Key:
H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.
Specificity / Sensitivity
Phospho-MKP1 (Ser359) (125E2) Rabbit mAb detects endogenous levels of MKP1 only when phosphorylated at serine 359.
Source / Purification
Monoclonal antibodies are produced by immunizing rabbits with a synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Ser359 of human MKP1.
Western Blotting
Western blot analysis of extracts from HeLa cells, treated with λ Phosphatase or ALLN, using Phospho-MKP1 (Ser359) (125E2) Rabbit mAb.
IP
Western blot analysis of immunoprecipitates from untreated or TPA-treated HeLa cells, using Phospho-MKP1 (Ser359) (125E2) Rabbit mAb.
Background
MAP kinases are inactivated by a family of dual-specificity protein phosphatases (DUSP) that differ in their substrate specificity, tissue distribution, inducibility by extracellular stimuli and cellular localization. MKP1 (MAP Kinase phosphatase 1) is primarily localized in the nucleus, and has broad substrate specificity towards p44/42 MAPK, p38 MAPK and SAPK/JNK (1-3). MKP1's transcription and activity are tightly regulated. First, MKP1 is transcriptionally induced through p44/42 MAPK, p53 and Jak2 (2,4,5). Second, MKP1 is phosphorylated by p44/42 MAPK at Ser359 and Ser364 in its carboxy-terminal region, which inhibits MKP1 degradation through the ubiquitin pathway (6).
- Sun, H. et al. (1993) Cell 75, 487-93.
- Brondello, J.M. et al. (1997) J Biol Chem 272, 1368-76.
- Franklin, C.C. and Kraft, A.S. (1997) J Biol Chem 272, 16917-23.
- Li, M. et al. (2003) J Biol Chem 278, 41059-68.
- Sandberg, E.M. et al. (2004) J Biol Chem 279, 1956-67.
- Brondello, J.M. et al. (1999) Science 286, 2514-7.
Application References
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Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.
