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Phospho-DUSP1/MKP1 (Ser359) (125E2) Rabbit mAb #2857

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP	H	Endogenous	40	Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

2857:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Phospho-DUSP1/MKP1 (Ser359) (125E2) Rabbit mAb detects endogenous levels of DUSP1 only when phosphorylated at serine 359.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser359 of human DUSP1.

Background

MAP kinases are inactivated by a family of dual-specificity protein phosphatases (DUSP) that differ in their substrate specificity, tissue distribution, inducibility by extracellular stimuli and cellular localization. DUSP1/MKP1 (MAP Kinase phosphatase 1) is primarily localized in the nucleus, and has broad substrate specificity towards p44/42 MAPK, p38 MAPK and SAPK/JNK (1-3). DUSP1's transcription and activity are tightly regulated. First, DUSP1 is transcriptionally induced through p44/42 MAPK, p53 and Jak2 (2,4,5). Second, DUSP1 is phosphorylated by p44/42 MAPK at Ser359 and Ser364 in its carboxy-terminal region, which inhibits DUSP1 degradation through the ubiquitin pathway (6).

1. Sun, H. et al. (1993) Cell 75, 487-93.
2. Brondello, J.M. et al. (1997) J Biol Chem 272, 1368-76.
3. Franklin, C.C. and Kraft, A.S. (1997) J Biol Chem 272, 16917-23.
4. Li, M. et al. (2003) J Biol Chem 278, 41059-68.
5. Sandberg, E.M. et al. (2004) J Biol Chem 279, 1956-67.
6. Brondello, J.M. et al. (1999) Science 286, 2514-7.

Application References

• Lee, W.H. et al. (2011) J Biol Chem 286, 21577-87. Applications: Western Blotting

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Companion Products

• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide
• 9101 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) Antibody
• 9102 p44/42 MAPK (Erk1/2) Antibody
• 9106 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (E10) Mouse mAb
• 4376 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (20G11) Rabbit mAb
• 4377 Phospho-p44/42 MAPK (Erk1/2) (Thr202/Tyr204) (197G2) Rabbit mAb
• 4695 p44/42 MAPK (Erk1/2) (137F5) Rabbit mAb
• 4696 p44/42 MAPK (Erk1/2) (L34F12) Mouse mAb

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

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For Research Use Only. Not For Use In Diagnostic Procedures.