Cell Signaling Technology

Product Pathways - Translational Control

eIF4GI Antibody #2858

Applications Reactivity Sensitivity MW (kDa) Source
W IHC-P IF-IC H M R Endogenous 220 Rabbit

Applications Key:  W=Western Blotting  IHC-P=Immunohistochemistry (Paraffin)  IF-IC=Immunofluorescence (Immunocytochemistry)
Reactivity Key:  H=Human  M=Mouse  R=Rat
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

eIF4GI Antibody detects endogenous levels of total eIF4GI protein.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the sequence of human eIF4GI. Antibodies are purified by peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of extracts from HepG2, RD and A204 cells using eIF4GI Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using eIF4GI Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lung carcinoma using eIF4GI Antibody.


IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human lymphoma using eIF4GI Antibody.

IHC-P (paraffin)

IHC-P (paraffin)

Immunohistochemical analysis of paraffin-embedded human breast carcinoma using eIF4GI Antibody in the presence of control peptide (left) or antigen-specific peptide (right).

IF-IC

IF-IC

Confocal immunofluorescent analysis of HeLa cells using eIF4GI Antibody (green). Actin filaments have been labeled with DY-554 phalloidin (red). Blue pseudocolor = DRAQ5® #4084 (fluorescent DNA dye).


Background

The initiation of translation is an important biological event and a variety of factors contribute to this process. Members of the eIF4 translation initiation factor family bind to the 5' m7GTP mRNA cap and unwind the mRNA secondary structure (1,2). The amino-terminal portion of eIF4G physically associates with eIF4E to stimulate the binding of eIF4E to the mRNA cap structure (3). eIF4G also interacts with eIF3 and eIF4A and serves as an adaptor molecule in the eIF4 complex (4). Moreover, eIF4G plays a role in internal ribosomal entry site (IRES)-mediated initiation of translation (5,6). The eIF4G family includes eIF4G1 (eIF4GI), eIF4G2 (p97, DAP5 or NAT1), and eIF4G3 (eIF4GII) (7). These factors share a homologous sequence that provides for interaction with initiation factors eIF3 and eIF4A. Both eIF4G1 and eIF4G3 are involved in cap-dependent translation, while eIF4G2 plays a role in IRES-mediated translation of some genes during cell stress (7,8).

  1. Yan, R. and Rhoads, R.E. (1995) Genomics 26, 394-398.
  2. Morley, S.J. et al. (1997) RNA 3, 1085-1104.
  3. Haghighat, A. and Sonenberg, N. (1997) J. Biol. Chem. 272, 21677-21680.
  4. De Gregorio, E. et al. (1998) RNA 4, 828-836.
  5. Ohlmann, T. et al. (1996) EMBO J. 15, 1371-1382.
  6. Borman, A.M. and Kean, K.M. (1997) Virology 237, 129-136.
  7. Henis-Korenblit, S. et al. (2002) Proc. Natl. Acad. Sci. USA 99, 5400-5405.
  8. Nevins, T.A. et al. (2003) J. Biol. Chem. 278, 3572-3579.

Application References

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

Products