Product Pathways - NF-kB Signaling

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Phospho-IκBα (Ser32) (14D4) Rabbit mAb #2859

Applications	Reactivity	Sensitivity	MW (kDa)	Isotype
W IP	H M R Mk (C) (B) (Dg) (Pg) (GP)	Endogenous	40	Rabbit IgG

Applications Key:  W=Western Blotting  IP=Immunoprecipitation
Reactivity Key:  H=Human  M=Mouse  R=Rat  Mk=Monkey  C=Chicken  B=Bovine  Dg=Dog  Pg=Pig  GP=Guinea Pig
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

2859:

Immunoprecipitation, Western Blotting

Specificity / Sensitivity

Phospho-IκBα (Ser32) (14D4) Rabbit mAb detects endogenous levels of IκBα only when phosphorylated at Ser32.

Source / Purification

Monoclonal antibody is produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser32 of human IκBα.

Background

The NF-κB/Rel transcription factors are present in the cytosol in an inactive state complexed with the inhibitory IκB proteins (1-3). Activation occurs via phosphorylation of IκBα at Ser32 and Ser36 followed by proteasome-mediated degradation that results in the release and nuclear translocation of active NF-κB (3-7). IκBα phosphorylation and resulting Rel-dependent transcription are activated by a highly diverse group of extracellular signals including inflammatory cytokines, growth factors, and chemokines. Kinases that phosphorylate IκB at these activating sites have been identified (8).

1. Baeuerle, P.A. and Baltimore, D. (1988) Science 242, 540-6.
2. Beg, A.A. and Baldwin, A.S. (1993) Genes Dev 7, 2064-70.
3. Finco, T.S. et al. (1994) Proc Natl Acad Sci USA 91, 11884-8.
4. Brown, K. et al. (1995) Science 267, 1485-8.
5. Brockman, J.A. et al. (1995) Mol Cell Biol 15, 2809-18.
6. Traenckner, E.B. et al. (1995) EMBO J 14, 2876-83.
7. Chen, Z.J. et al. (1996) Cell 84, 853-62.
8. Karin, M. and Ben-Neriah, Y. (2000) Annu Rev Immunol 18, 621-63.

Application References

• Shembade, N. et al. (2007) J Virol 81, 13735-42. Applications: Western Blotting
• Yang, Y.J. et al. (2008) J Biol Chem 283, 32110-8. Applications: Western Blotting
• Taura, M. et al. (2008) Mol Cell Biol 28, 6557-67. Applications: Western Blotting
• Wang, H. et al. (2009) J Immunol 183, 4755-63. Applications: IHC-F (floating)
• Xie, S. et al. (2010) J Immunol 184, 2289-96. Applications: Western Blotting

Have you published research involving the use of our products? If so we'd love to hear about it. Please let us know!

Companion Products

• 9242 IκBα Antibody
• 9243 NF-κB Control Cell Extracts
• 9246 Phospho-IκBα (Ser32/36) (5A5) Mouse mAb
• 9247 IκBα (112B2) Mouse mAb (Carboxy-terminal Antigen)
• 4812 IκBα (44D4) Rabbit mAb
• 4814 IκBα (L35A5) Mouse mAb (Amino-terminal Antigen)
• 9936 NF-κB Pathway Sampler Kit
• 7071 Phototope^®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
• 7074 Anti-rabbit IgG, HRP-linked Antibody
• 7720 Prestained Protein Marker, Broad Range (Premixed Format)
• 7727 Biotinylated Protein Ladder Detection Pack
• 7003 20X LumiGLO^® Reagent and 20X Peroxide

Rabbit Monoclonals Produced Using Epitomics® Technology, U.S. Patent No. 5,675,063.

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For Research Use Only. Not For Use In Diagnostic Procedures.