Product Pathways - Tyrosine Kinase / Adaptors
Phospho-c-Abl (Tyr245) Antibody #2861
PhosphoSitePlus® protein, site, and accession data: Abl
| Applications | Reactivity | Sensitivity | MW (kDa) | Source |
|---|---|---|---|---|
| W | H (M) | Endogenous | 135 (c-Abl); 210 (Bcr-Abl) | Rabbit |
Applications Key:
W=Western Blotting
Reactivity Key:
H=Human
M=Mouse
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.
Protocols
- 2861:
- Western Blotting
Specificity / Sensitivity
Phospho-c-Abl (Tyr245) Antibody detects endogenous levels of c-Abl only when phosphorylated at tyrosine 245. The antibody cross-reacts with activated EGF receptors and PDGF receptors.
Source / Purification
Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr245 of human c-Abl.
Western Blotting
Western blot analysis of extracts from K562 cells, untreated or treated with an Abl inhibitor, using Phospho-c-Abl (Tyr245) Antibody.
Background
The c-Abl proto-oncogene encodes a nonreceptor protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion, and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation; PDGF stimulation; and binding to c-Jun, Nck, and RFX1 (2,4). The in vivo mechanism for regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation at Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation at Tyr412, which is located in the kinase activation loop of c-Abl, is required for kinase activity (6).
- Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
- Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182.
- Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
- Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16.
- Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
- Pluk, H. et al. (2002) Cell 108, 247-259.
Application References
- Ganapathipillai, S.S. et al. (2008) Cancer Res 68, 5769-77. Applications: Western Blotting
- Goss, V.L. et al. (2006) Blood 107, 4888-97. Applications: Western Blotting
- Smith, K. M. et al. (2003) Autoinhibition of Bcr-Abl through its SH3 domain. Mol. Cell 12, 27-37. Applications: Western Blotting
- Komatsu, N. et al. (2002) A member of Forkhead Transcription Factor FKHRL1 Is a Downstream Effector of STI571-induced Cell Cycle Arrest in BCR-ABL-expressing Cells. J.Biol.Chem. 278 (8), 6411-6419. Applications: Western Blotting
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Companion Products
- 2865 Phospho-c-Abl (Tyr412) (247C7) Rabbit mAb
- 2868 Phospho-c-Abl (Tyr245) (73E5) Rabbit mAb
- 3009 Phospho-c-Abl (Tyr204) (C42B5) Rabbit mAb
- 2862 c-Abl Antibody
- 2864 Phospho-c-Abl (Thr735) Antibody
- 3098 Phospho-c-Abl (Tyr89) (61A6) Rabbit mAb
- 7071 Phototope®-HRP Western Blot Detection System, Anti-rabbit IgG, HRP-linked Antibody
- 7074 Anti-rabbit IgG, HRP-linked Antibody
- 7720 Prestained Protein Marker, Broad Range (Premixed Format)
- 7727 Biotinylated Protein Ladder Detection Pack
- 7003 20X LumiGLO® Reagent and 20X Peroxide
For Research Use Only. Not For Use In Diagnostic Procedures.
