Cell Signaling Technology

Product Pathways - Adhesion

Plectin-1 Antibody #2863

Applications Reactivity Sensitivity MW (kDa) Source
W H Mk Endogenous 400-500 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human  Mk=Monkey
Species cross-reactivity is determined by western blot. Species enclosed in parentheses are predicted to react based on 100% sequence homology.

Protocols

Specificity / Sensitivity

Plectin-1 Antibody detects endogenous levels of total plectin-1 protein. The antibody is expected to cross-react with all isoforms of plectin-1 based on the sequence of the immunogenic peptide.

Source / Purification

Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the central sequence of human plectin-1.

Western Blotting

Western Blotting

Western blot analysis of extracts from various cell types using Plectin-1 Antibody. Molecular weights were determined using a pre-stained molecular weight marker (not shown).

Background

Plectin is a large, widely expressed protein that crosslinks the intermediate filament and actin cytoskeleton, mechanically stabilizing cells and tissues. Plectin also plays a role in the regulation of actin dynamics and acts as a scaffold for signaling molecules (1). Plectin is important in the stabilization of hemidesmosomes, crosslinking them to the intermediate filament network. Research studies have shown that mutations in plectin and other genes coding for hemidesmosomal proteins can cause epidermolysis bullosa, a condition manifested by fragile skin and frequent blistering (1,2). Plectin modulates signals to PKC through binding and sequestration of RACK1, the receptor for activated C kinase 1 (3,4). Plectin is also involved in the regulation of cytokeratin architecture and cell stress response (4), signaling through the chemokine receptor CXCR4 (5) and regulation of AMP-activated protein kinase (AMPK) activity and signaling in mouse myotubes (6).

  1. Wiche, G. (1998) J Cell Sci 111 ( Pt 17), 2477-86.
  2. Pfendner, E. et al. (2005) Exp Dermatol 14, 241-9.
  3. Osmanagic-Myers, S. and Wiche, G. (2004) J Biol Chem 279, 18701-10.
  4. Osmanagic-Myers, S. et al. (2006) J Cell Biol 174, 557-68.
  5. Ding, Y. et al. (2008) Exp Cell Res 314, 590-602.
  6. Gregor, M. et al. (2006) J Cell Sci 119, 1864-75.

Application References

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Companion Products

For Research Use Only. Not For Use In Diagnostic Procedures.

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