Cell Signaling Technology

Product Pathways - Tyrosine Kinase/ Adaptors

Phospho-c-Abl (Thr735) Antibody #2864

Applications Reactivity MW (kDa) Source
W H 135 Rabbit

Applications Key:  W=Western Blotting
Reactivity Key:  H=Human
Species enclosed in parentheses are predicted to react based on 100% sequence homology. Species cross-reactivity is determined by Western blot.

Specificity / Sensitivity

Phospho-c-Abl (Thr735) Antibody detects endogenous levels of c-Abl or Bcr-Abl only when phosphorylated at threonine 735. The antibody does not cross-react with other related proteins.

Source / Purification

Polyclonal antibodies are produced by immunizing rabbits with synthetic phospho-peptide (KLH-coupled) corresponding to residues surrounding Thr735 of human c-Abl. Antibodies are purified by protein A and peptide affinity chromatography.

Western Blotting

Western Blotting

Western blot analysis of immunoprecipitated samples from HeLa cells treated with 0.5 M sorbitol for 30 minutes, using Phospho-c-Abl (Thr735) Antibody (upper) or c-Abl Antibody #2862 (lower).

Background

The c-Abl proto-oncogene encodes a nonreceptor type protein tyrosine kinase that is ubiquitously expressed and highly conserved in metazoan evolution. c-Abl protein is distributed in both the nucleus and the cytoplasm of cells. It is implicated in regulating cell proliferation, differentiation, apoptosis, cell adhesion and stress responses (1-3). c-Abl kinase activity is increased in vivo by diverse physiological stimuli including integrin activation, PDGF stimulation and binding to c-Jun, Nck and RFX1 (2,4). The in vivo mechanism of regulation of c-Abl kinase activity is not completely understood. Tyr245 is located in the linker region between the SH2 and catalytic domains. This positioning is conserved among Abl family members. Phosphorylation of Tyr245 is involved in the activation of c-Abl kinase (5). In addition, phosphorylation of Tyr412 which is located in the kinase activation loop of c-Abl is required for kinase activity (6). Thr735 is located within a conserved 14-3-3 protein binding motif region, and can be phosphorylated upon stress stimulation or TPA treatment (Wu, J. et al. unpublished data). Phosphorylation at Thr735 may play an important role in regulating c-Abl localization as well as its function.

  1. Wang, J.Y. et al. (2000) Oncogene 19, 5643-5650.
  2. Van Etten, R.A. et al. (1999) Trends Cell. Biol. 9, 179-182.
  3. Danial, N.N. et al. (2000) Oncogene 19, 2523-2531.
  4. Shaul, Y. et al. (2000) Cell Death Differ. 7, 10-16.
  5. Brasher, B.B. et al. (2000) J. Biol. Chem. 275, 35631-35637.
  6. Pluk, H. et al. (2002) Cell 108, 247-259.

Application References

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